FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004724411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25515312
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTCTACTCTCAGTTTACTGCTAAAT8942203.5046406643979116No Hit
CGTAGCGACGGGCGGTGTGTACAAAGGG3815121.4952276499695556No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1495270.586028499279178No Hit
GCACTTACTGGGAATTCCTCGTTCATGG1440930.5647314835891484No Hit
TTTGCGCTGTTATCCCTAGGGTAACTTG1121790.4396536479742047No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT993370.3893230856828245No Hit
GACGCGGGCTTATGCGGAGGAGAATGTT978970.3836794157171192No Hit
TGCGTGGGGGTTTTAGTTAAATGTCCTT959370.37599775381935363No Hit
TGCGTGGGTACTTGCGCTTACTTTGTAG940540.36861787149614317No Hit
TTTGCGCTTACTTTGTAGCCTTCATCAG826880.32407207091961093No Hit
GCGCAGTTATCCCTAGGGTAACTTGTTC814570.31924751694198367No Hit
CGCTTCAGGGCCCTGTTCAACTAAGCAC771790.30248111408553424No Hit
TGTGTTTTAATCTGACGCAGGCTTATGC664960.26061213752745804No Hit
CGTAGGACTTTAATCGTTGAACAAACGA627210.2458170999437514No Hit
TGCGTGGGTATAATACTAAGTTGAGATG614480.24082793892545779No Hit
ACACGACGCTCTTCCGATCTACACGACG598170.23443569884624574No Hit
GCACTCTACTGGGAATTCCTCGTTCATG565770.22173744142340882No Hit
GCTTGAACGCTTTCTTAATTGGTGGCTG532410.20866293933619157No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG531060.20813384527690668No Hit
CGTAGGGGTTTTAGTTAAATGTCCTTTG477840.18727578169532083No Hit
AAGGCAGGGACTTAATCAACGCAAGCTT437790.17157932460320297No Hit
CACTCCAGTTTACTGCTAAATCCACCTT380040.14894585651157236No Hit
CGTGCGTTATCGGAATTAACCAGACAAA368190.14430158643562735No Hit
AAGGCAGAATCCAGATGCTCAAGGCCCT354580.13896753447498505No Hit
CGACCTTTGAAGTATACTTGAGGAGGGT326170.127833043938479No Hit
GTCTCGTTCATGGGGAATAATTGCAATC319020.12503080503189615No Hit
AGAGCTTGAACGCTTTCTTAATTGGTGG315920.12381584830316791No Hit
CACAGGCTTATGCGGAGGAGAATGTTTT296490.11620081306471973No Hit
CGACCTTTCCTTATGAGCATGCCTGTGT282890.11087068031933138No Hit
GTAGCGACGGGCGGTGTGTACAAAGGGC280870.11007899883803106No Hit
CGTAGCGGCTGCACCATCGGGATGTCCT268860.10537202131802269No Hit
CGTAGCGCGCGTGCAGCCCCGGACATCT265490.10405124577743748No Hit
GCACTCTAGAATAGGATTGCGCTGTTAT264570.10369067797407298No Hit
GCACTCTAATTTTTTCAAAGTAAACGCT264050.10348687878086696No Hit
TGGTTGATTGTAGATATTGGGCTGTTAA263810.10339281761477187No Hit
CGTAGCGTTCTGGCGAGCAGTTTTGTTG263670.1033379486012164No Hit
ATTGGACTCTAGAATAGGATTGCGCTGT256580.10055922498615733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)