FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004724413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70990463
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCCAATGGATCCTCGTTAAAGGA3093300.4357345859259997No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA2780100.39161598368501976No Hit
TGACTTTTACTTCCTCTAGATAGTCAAG1729110.24356933691219904No Hit
AGGGTGATAGATTGGTCCAATTGGGTGT1700810.23958288594342594No Hit
AGTGAGGGTAATAATGACTTGTTGGTTG1637330.23064083974209323No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1540650.2170221090120232No Hit
TACGGGCGGTGTGTACAAAGGGCAGGGA1485890.20930839681944322No Hit
AGTGAGGAGTTCAGTTATATGTTTGGGA1376780.19393872667093326No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG1269960.17889163506371272No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1187950.16733937909378052No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAA1178300.16598004157262644No Hit
CATGCCTGTGTTGGGTTGACAGTGAGGG870020.1225544901714474No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT839640.11827504209966909No Hit
AGTTGGTTGATTGTAGATATTGGGCTGT826430.11641422876760221No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA801830.11294897456859804No Hit
CGTTCGTTATCGGAATTAACCAGACAAA791480.11149103225316336No Hit
CACCTTGTTACGACTTTTACTTCCTCTA783630.11038524991730228No Hit
GGATTACTCCGGTCTGAACTCAGATCAC776670.10940483653416938No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTT748800.10547895708188294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)