FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004724425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87701965
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGACGCTCTTCCGATCTACACGACGCT12586251.4351160775017981Illumina Single End PCR Primer 1 (95% over 23bp)
GGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGC4585370.5228354917703383No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA3547060.4044447578797123No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG2912470.3320872000986523No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC2786500.31772378190158No Hit
ACACGACGCTCTTCCGATCTACACGACGCTCTTCCGATCTACACGACGCC2511300.2863447814424683Illumina Single End PCR Primer 1 (95% over 23bp)
TACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGA2511040.28631513558447635No Hit
CAGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCT2389060.27240666728504886No Hit
AGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAA2176830.24820766558651225No Hit
TGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCG2085670.23781337168443145No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA2032080.23170290426217932No Hit
AGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGA1979510.22570873982127995No Hit
CCCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGT1866420.2128139318201137No Hit
CTCTACGAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1529520.17439974121446425No Hit
CCCTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGG1528690.17430510251395165No Hit
GTACTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAG1175740.13406085028995646No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT933640.10645599559827423No Hit
ACACGACGCTCTTCCGATCTACACGACGCTCTTCCGATCTACACGACGCA926810.10567722171333334Illumina Single End PCR Primer 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)