FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52329082
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT12251492.3412392367211794No Hit
TGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC5874521.122610941273535No Hit
GCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATA3373540.6446778485431868No Hit
TGACTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC2553860.48803837223821356No Hit
AGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCC2148520.4105785765551935No Hit
ACACGACGCTCTTCCGATCTACACGACGCTCTTCCGATCTACACGACGCT2084630.39836930447203334Illumina Single End PCR Primer 1 (95% over 23bp)
CAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTT2070620.39569201691709405No Hit
GCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGT1714120.32756546350268484No Hit
AGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTG1600980.3059445988370291No Hit
CATCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAA1507920.2881609885684599No Hit
TGGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC1489450.2846314024771159No Hit
TTTCCTCTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGA1410360.2695174358304241No Hit
CCACCTACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG1369220.2616556506762339No Hit
AGCTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAG1263710.24149286624214045No Hit
AGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGG1261790.24112595745516804No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACC1216240.2324214286809006No Hit
GCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG1086170.20756526934678504No Hit
CGACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAG1024170.19571717310080083No Hit
CTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACA1014890.19394378063043416No Hit
CGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG998460.19080403512524832No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT989290.18905166347080196No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG963010.184029599449117No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA894120.17086483573321617No Hit
AGTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTG891710.17040428876623517No Hit
CGACTTCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCAT845690.16160994377849014No Hit
CATGCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAG841270.16076528917514737No Hit
AGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATA828310.1582886548630836No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT820480.15679235496621172No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAAT788320.15064663278442378No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC788130.15061032410237962No Hit
TGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC770910.1473196109192208No Hit
CATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGAT766100.14640042796852426No Hit
AGGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTC760910.14540862765373946No Hit
ATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTA722890.13814306927837947No Hit
GGGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT677330.12943662952084656No Hit
GCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGG676680.12931241560859028No Hit
CATGCCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT643270.12292782051861716No Hit
ATCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAG642970.12287049102065273No Hit
CAGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCG566280.10821516035767645No Hit
CGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCA556700.10638443838934533No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGAC547630.10465117656755377No Hit
CCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGG544770.10410463535362612No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG533630.10197579999587991No Hit
GCTTCCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGC532560.10177132478647341No Hit
CCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATT532340.10172928315463282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGT2227200.068.129193
TAAGGAC1589900.067.90566
CGTATGA2182000.067.775777
CACGTAT2193100.067.600295
ACACGTA2193300.067.586424
CCTACAC3162550.067.4950561
ACGTATG2197250.067.3085566
TATGAAA2209600.067.210999
GTATGAA2264000.067.076988
CTAAGGA1453150.066.97475
ATGAAAC2236150.066.5788910
GACAGAA1635050.066.20743610
AATCTAT659650.065.9178546
TATGGGG19145800.065.6202334
CTTATAT28526100.065.4683330
GCTAATC671050.065.459423
TCTTATA28502050.065.4583329
TTCTTAT28718050.065.3932628
AGGACAG1669100.065.381648
TGAAACG391750.065.3573611
Produced by FastQC (version 0.11.5)