FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122330562
Sequences flagged as poor quality0
Sequence length28
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACCAC18262571.4928869533028057No Hit
CCACCAACTAAGAACGGCCATGCACCAC9973380.8152811396386784No Hit
CTCGACTGCCGGCGACGGCCGGGTATGG9723480.7948528839424445No Hit
ATCCACCAACTAAGAACGGCCATGCACC9659490.7896219752509598No Hit
CTACACCTCTCATGTCTCTTCACCGTGC9159510.7487507496286986No Hit
CGTTCGTTATCGGAATTAACCAGACAAA8782800.7179563190431514No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA8770720.716968830732585No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA8748450.7151483535242812No Hit
CGTCACTCTCGACTGCCGGCGACGGCCG7219810.5901885744626924No Hit
TGTCCACTCTCGACTGCCGGCGACGGCC4452130.3639425771623611No Hit
CGACTTCCGGCGACGGCCGGGTATGGGC3992130.3263395454686131No Hit
CGTCCATTCTCGACTGCCGGCGACGGCC3826620.3128098111737605No Hit
GCACTCTCGACTGCCGGCGACGGCCGGG3601860.2944366429053109No Hit
TGCCCTACAATGGATCCTCGTTAAAGGA3480860.28454541065543376No Hit
GCTCCACCAACTAAGAACGGCCATGCAC3462600.2830527337886341No Hit
AGCTCTCGACTGCCGGCGACGGCCGGGT3374870.27588118167886777No Hit
GCAGCAACTTTAATATACGCTATTGGAG3206090.2620841388761052No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT3187430.2605587637208762No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAA3166250.2588273893485424No Hit
CGTCTACTCTCGACTGCCGGCGACGGCC3125320.2554815369850095No Hit
CTCTCGACTGCCGGCGACGGCCGGGTAT3083090.2520294151840813No Hit
ACACCAACTAAGAACGGCCATGCACCAC2945930.24081717208165854No Hit
TGCCCTAGAGCCAATCCTTATCCCGAAG2854600.23335133537602812No Hit
CCACTCTCGACTGCCGGCGACGGCCGGG2473520.20219967598939012No Hit
CTACACCGTGCCAGACTAGAGTCAAGCT2440270.19948163076370073No Hit
AACCGCGGCTGCTGGCACCAGACTTGCC2383950.19487771175284882No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTA2232560.18250222703955205No Hit
CTCGTACTGAGCAGGATTACCATGGCAA2220180.1814902150126638No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG2164000.17689774040276213No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA1929230.15770629746636822No Hit
CGACTGCCGGCGACGGCCGGGTATGGGC1923580.1572444341423037No Hit
ACGGCCATGCACCACCACCCACGGAATC1749960.14305174204954607No Hit
CCGTTCACTCTCGACTGCCGGCGACGGC1716670.14033042699501372No Hit
ACCCACTCTCGACTGCCGGCGACGGCCG1701820.13911650303707426No Hit
AACCGCGGGAACCCCGCATCTTCACGGG1690650.13820340333268477No Hit
GTGGGTCGCGTAACTAGTTAGCATGCCA1608210.13146428608739655No Hit
TGCCAAAGTCTCGTTCGTTATCGGAATT1571310.12844786897978935No Hit
GGGTTGCGTAACTAGTTAGCATGCCAGA1565060.12793695822308082No Hit
CCACTAACTAAGAACGGCCATGCACCAC1562780.12775057797903355No Hit
ACACCTCTCATGTCTCTTCACCGTGCCA1495690.1222662575522215No Hit
CCTACCAACTAAGAACGGCCATGCACCA1455260.11896127804922534No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1437750.1175299104732307No Hit
ACACCCTCATGTCTCTTCACCGTGCCAG1435950.11738276817529866No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA1399380.1143933271556457No Hit
TGTTCGTTATCGGAATTAACCAGACAAA1376620.11253279454401591No Hit
CTCGTACTGCCGGCGACGGCCGGGTATG1315940.10757246418928411No Hit
CTACCAACTAAGAACGGCCATGCACCAC1286860.10519529862047065No Hit
CGCTTCACCAACTAAGAACGGCCATGCA1278220.10448901559039678No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1269190.10375085172910431No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGC1250100.10219032591381376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCTA4387200.021.1834681
AACCGCG947250.020.8259641
CACCCAA1409750.020.7327294
TGCCCTA1797200.020.6968921
CACCTAC2325150.020.5855642
TACACCC1513850.020.425022
CTACTAA2050950.020.3898245
GCCCTAG938800.020.3847732
TAGACGC447000.020.3201057
ACCTACT2078600.020.2919543
AGCGCAA451650.020.276510
CCACTAC1190700.020.2031271
TAGTAGA808150.020.1989946
CTAGTAG847400.020.1942655
ACCTAGT1189450.020.1754323
ATGGACG457650.020.12602810
TACTAAG2079900.020.1034746
TAGATTA879850.020.0630959
ACCCAAG1444500.020.0527295
CCTAGTA998350.020.0375734
Produced by FastQC (version 0.11.5)