FastQCFastQC Report
Thu 29 Sep 2022
EGAF00004724434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122330562
Sequences flagged as poor quality0
Sequence length89
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12277581.0036396301359263Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6033950.49324959367063154No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG2601380.21265168388583058No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT2582940.21114429278923774No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC2507340.20496431627609135No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2249260.18386738058147725Clontech SMART CDS Primer II A (100% over 24bp)
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTAT2126400.1738241012904036No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT2080310.17005644100613224No Hit
GCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGG1860540.15209118388583878No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1856420.1517543915150165No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1849540.15119198095403175No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG1839430.15036553171398004No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT1832740.1498186528399992No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGG1637910.1338921340032755No Hit
GTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCC1628000.13308203390743845No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG1580390.12919012012713554No Hit
CCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTC1563570.12781515709868152No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG1528420.12494179500295273No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA1525270.12468429598157164No Hit
GGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA1519300.12419627402676366No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC1511370.12354803045865187No Hit
GAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCC1500700.12267580361479904No Hit
GTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGC1499530.12258016112114323No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG1475210.12059210518463898No Hit
GGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGC1457070.11910923780436813No Hit
GTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCC1443800.11802447208572459No Hit
GCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAAC1431020.11697976177040698No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTA1428800.1167982862696241No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG1418470.11595385297093624No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCG1411700.11540043443926955No Hit
GGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTC1374380.11234968412881156No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG1356910.11092158638165987No Hit
CTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAA1326580.10844223866150471No Hit
GTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTC1326120.10840463562981097No Hit
GGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT1315470.10753404370037964No Hit
CAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTC1306670.10681468135493402No Hit
GCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTG1299540.10623183436368093No Hit
GGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCA1278180.10448574576155385No Hit
GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTC1276940.10438438106742287No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG1258580.10288352962851588No Hit
AATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGG1244460.10172928004695998No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC1228230.10040254699393925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)