FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004724435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118495151
Sequences flagged as poor quality0
Sequence length28
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACCAC17639891.488659227920643No Hit
CCACCAACTAAGAACGGCCATGCACCAC9673860.8163929003305797No Hit
CTCGACTGCCGGCGACGGCCGGGTATGG9415720.7946080426531545No Hit
ATCCACCAACTAAGAACGGCCATGCACC9353080.7893217503896004No Hit
CTACACCTCTCATGTCTCTTCACCGTGC8850990.7469495523913886No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA8517610.7188150677996942No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA8509030.7180909875375406No Hit
CGTTCGTTATCGGAATTAACCAGACAAA8486500.7161896439120956No Hit
CGTCACTCTCGACTGCCGGCGACGGCCG7027310.5930462082790207No Hit
TGTCCACTCTCGACTGCCGGCGACGGCC4322290.36476513709831043No Hit
CGACTTCCGGCGACGGCCGGGTATGGGC3887490.32807165248475023No Hit
CGTCCATTCTCGACTGCCGGCGACGGCC3713140.3133579702345795No Hit
GCACTCTCGACTGCCGGCGACGGCCGGG3493620.2948323176532346No Hit
TGCCCTACAATGGATCCTCGTTAAAGGA3390630.2861408227582241No Hit
GCTCCACCAACTAAGAACGGCCATGCAC3344430.2822419290389359No Hit
AGCTCTCGACTGCCGGCGACGGCCGGGT3265160.2755522038197158No Hit
GCAGCAACTTTAATATACGCTATTGGAG3116300.2629896644462692No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT3096020.2612782020084518No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAA3066050.2587489845892512No Hit
CGTCTACTCTCGACTGCCGGCGACGGCC3036280.2562366454978398No Hit
CTCTCGACTGCCGGCGACGGCCGGGTAT2986320.2520204392161161No Hit
ACACCAACTAAGAACGGCCATGCACCAC2849070.2404376867708283No Hit
TGCCCTAGAGCCAATCCTTATCCCGAAG2780430.23464504467360017No Hit
CCACTCTCGACTGCCGGCGACGGCCGGG2392260.2018867421840747No Hit
CTACACCGTGCCAGACTAGAGTCAAGCT2376750.20057782786402795No Hit
AACCGCGGCTGCTGGCACCAGACTTGCC2309720.19492105630550233No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTA2166110.18280157303652028No Hit
CTCGTACTGAGCAGGATTACCATGGCAA2148610.18132471935497174No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG2090210.1763962476405469No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA1874970.15823179127388934No Hit
CGACTGCCGGCGACGGCCGGGTATGGGC1873720.1581263017252073No Hit
ACGGCCATGCACCACCACCCACGGAATC1688700.14251216068748668No Hit
CCGTTCACTCTCGACTGCCGGCGACGGC1671750.14108172240735825No Hit
ACCCACTCTCGACTGCCGGCGACGGCCG1644110.13874913750690102No Hit
AACCGCGGGAACCCCGCATCTTCACGGG1632310.13775331616734257No Hit
GTGGGTCGCGTAACTAGTTAGCATGCCA1555010.13122984247684533No Hit
TGCCAAAGTCTCGTTCGTTATCGGAATT1530290.12914368116210934No Hit
GGGTTGCGTAACTAGTTAGCATGCCAGA1513200.1277014280525285No Hit
CCACTAACTAAGAACGGCCATGCACCAC1510460.12747019496181747No Hit
ACACCTCTCATGTCTCTTCACCGTGCCA1441490.12164970362373731No Hit
CCTACCAACTAAGAACGGCCATGCACCA1403760.11846560708631867No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1396430.11784701637284718No Hit
ACACCCTCATGTCTCTTCACCGTGCCAG1395140.11773815115860733No Hit
TGTTCGTTATCGGAATTAACCAGACAAA1346720.11365190800085988No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA1341540.11321475931112152No Hit
CTCGTACTGCCGGCGACGGCCGGGTATG1265650.1068102778315376No Hit
CTACCAACTAAGAACGGCCATGCACCAC1249310.10543131845116599No Hit
CGCTTCACCAACTAAGAACGGCCATGCA1233260.10407683264608862No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1230670.10385825830121942No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGC1212650.10233752096741916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)