FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98457645
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACCAC16957501.7223141991665551No Hit
CCACCAACTAAGAACGGCCATGCACCAC7444510.7561129458255883No Hit
ATCCACCAACTAAGAACGGCCATGCACC6064360.6159359184347747No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA5575150.5662485630242323No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT4495070.45654860016202903No Hit
AACCGCGGGAACCCCGCATCTTCACGGG4199260.426504208992608No Hit
CCACTACCCATTACCAGATCGCTTCACA3980270.40426215760086487No Hit
ACACGACGCTCTTCCGATCTACACGACG3854770.3915155598125468No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA3587230.36434245405727506No Hit
CGTTCGTTATCGGAATTAACCAGACAAA3063720.3111713671396467No Hit
ACACCAACTAAGAACGGCCATGCACCAC2642140.26835295522252234No Hit
CGCTTCACCAACTAAGAACGGCCATGCA2346470.23832278336537502No Hit
TGTCCACTCTCGACTGCCGGCGACGGCC2216660.22513843389205582No Hit
GACGGCTCTATGAAATCTTATCTCGAGG2037190.20691029122217988No Hit
AGTGAGGGTAATAATGACTTGTTGGTTG2013620.20451636843436585No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1999000.20303146596691402No Hit
CGTCACTCTCGACTGCCGGCGACGGCCG1860510.1889655191326179No Hit
AGTGAGGAGTTCAGTTATATGTTTGGGA1847300.18762382545306663No Hit
GCTCCACCAACTAAGAACGGCCATGCAC1800630.1828837161400722No Hit
CTCCTAGATTCAAGCGATGCAGTCTTAA1648550.16743748035005307No Hit
AGGGTGATAGATTGGTCCAATTGGGTGT1610420.16356474908576168No Hit
CCACGACCCATTACCAGATCGCTTCACA1513840.1537554549471501No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT1494180.1517586572378407No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAA1487830.15111370985970668No Hit
CGTCCATTCTCGACTGCCGGCGACGGCC1465890.14888534049336646No Hit
TCCACACCCATTACCAGATCGCTTCACA1464710.14876549200420142No Hit
CCTGGATTCAAGCGATGCAGTCTTAAAG1403730.14257196584378998No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG1292570.1312818318983762No Hit
CTCTACGACCCATTACCAGATCGCTTCA1191450.12101142577602786No Hit
CGTCTACTCTCGACTGCCGGCGACGGCC1187480.1206082067065488No Hit
CCGTTCACTCTCGACTGCCGGCGACGGC1179570.1198048155630779No Hit
CTCCTGGATTCAAGCGATGCAGTCTTAA1108730.11260984355252456No Hit
GACGGCTTTTGGAGATTAGCTCACCCTT1048770.10651991523867954No Hit
CCACTCTCGACTGCCGGCGACGGCCGGG1009270.10250803784713722No Hit
CACCAACTAAGAACGGCCATGCACCACC992990.10085453496272431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCTA4077950.021.467911
CACCTAC2043900.020.9289512
ACCTAGT1907250.020.8554483
TCCGTGA1625200.020.8396459
ACGCCAG1227250.020.7966941
TAGTCCG1634550.020.7036156
CTACTAA1894100.020.7019945
TGAGGTC744250.020.6349833
ACGGGCT1842900.020.5167561
CCGTGAC1659700.020.39452610
TGGCTGG685450.020.3394951
ACCCACT1052800.020.2494871
CTAGTCC1676500.020.2100545
CCACTAC1368600.020.1750531
GGCGACT281100.020.1551281
ACCTACT1961100.020.0914463
CCTACTA1961250.020.0767614
ATCTACA507450.020.06269817
GAGGTCT668500.020.0583134
AACCGCG731400.020.035221
Produced by FastQC (version 0.11.5)