FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004724438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98457645
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5599530.568724754690202Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4359190.4427477419351235No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1771270.17990172322321948No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1683980.17103598202049217No Hit
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTAT1413520.14356630203779505No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT1409630.1431712082896153No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1257460.1277158315131344No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG1251840.12714502769185673No Hit
GTCGCCTTGGTGCGCCTTTACCACACCAACTAGCTAATCCTACGCGGGCT1103570.112085760328718No Hit
GTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCC1100800.11180442107872883No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG1097900.11150987818162825No Hit
GGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA1066090.10827904729998367No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC1034440.10506446706093772No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG1027350.10434436046078495No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA1015000.10309001398520144No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG1007400.10231810846176546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)