FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95340444
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACCAC16415731.7218012955760937No Hit
CCACCAACTAAGAACGGCCATGCACCAC7221000.7573910606080249No Hit
ATCCACCAACTAAGAACGGCCATGCACC5863950.6150537750799651No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA5390140.565357132173624No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT4371130.4584759433257936No Hit
AACCGCGGGAACCCCGCATCTTCACGGG4056540.4254794533996506No Hit
CCACTACCCATTACCAGATCGCTTCACA3836820.4024336198811912No Hit
ACACGACGCTCTTCCGATCTACACGACG3565960.37402385078047257No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA3475290.3645137209556104No Hit
CGTTCGTTATCGGAATTAACCAGACAAA2981280.31269835496046144No Hit
ACACCAACTAAGAACGGCCATGCACCAC2559900.26850095222967496No Hit
CGCTTCACCAACTAAGAACGGCCATGCA2268650.23795253145663975No Hit
TGTCCACTCTCGACTGCCGGCGACGGCC2153230.22584644141157975No Hit
GACGGCTCTATGAAATCTTATCTCGAGG1967940.20641187699944005No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1941650.203654390365541No Hit
AGTGAGGGTAATAATGACTTGTTGGTTG1931320.20257090474636347No Hit
CGTCACTCTCGACTGCCGGCGACGGCCG1810190.18986590832322955No Hit
AGTGAGGAGTTCAGTTATATGTTTGGGA1785560.18728253457682661No Hit
GCTCCACCAACTAAGAACGGCCATGCAC1747740.18331569758580105No Hit
CTCCTAGATTCAAGCGATGCAGTCTTAA1601190.16794446646378108No Hit
AGGGTGATAGATTGGTCCAATTGGGTGT1558450.1634615840471647No Hit
CCACGACCCATTACCAGATCGCTTCACA1469440.15412556710979866No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT1445280.15159149038575906No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAA1445010.15156317081972054No Hit
TCCACACCCATTACCAGATCGCTTCACA1417730.1487018457770136No Hit
CGTCCATTCTCGACTGCCGGCGACGGCC1406860.147561721025759No Hit
CCTGGATTCAAGCGATGCAGTCTTAAAG1355920.14221876289982455No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG1243440.13042104146273956No Hit
CGTCTACTCTCGACTGCCGGCGACGGCC1148410.1204536030899961No Hit
CTCTACGACCCATTACCAGATCGCTTCA1142670.11985155009347345No Hit
CCGTTCACTCTCGACTGCCGGCGACGGC1142490.11983267038278109No Hit
CTCCTGGATTCAAGCGATGCAGTCTTAA1070470.11227868836020945No Hit
GACGGCTTTTGGAGATTAGCTCACCCTT1000210.1049093079532963No Hit
CCACTCTCGACTGCCGGCGACGGCCGGG976560.10242872374288503No Hit
CACCAACTAAGAACGGCCATGCACCACC959910.10068235050384285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCTA3952150.021.5029351
CACCTAC1980450.020.9960482
ACCTAGT1856550.020.850413
TCCGTGA1582950.020.8478439
ACGCCAG1199300.020.8220651
CTACTAA1837350.020.7360535
TAGTCCG1593050.020.7018576
TGAGGTC716900.020.574123
ACGGGCT1782850.020.4983961
CCGTGAC1616050.020.4058610
TGGCTGG648400.020.3801141
GGCGACT265600.020.3272381
ACCCACT1005650.020.2257441
CTAGTCC1637450.020.1727475
CCACTAC1301750.020.1546751
CCTACTA1901800.020.101584
ACCTACT1902000.020.0930023
ATCTACA470000.020.09120817
GAGGTCT643150.020.0649224
TGCGGGT487250.019.9699761
Produced by FastQC (version 0.11.5)