FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004724440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95340444
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5417630.5682404835454721Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4184750.4389270517766835No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1715650.1799498647184819No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1627690.17072397942682122No Hit
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTAT1363200.1429823423100484No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT1362510.14290997008572773No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA1215130.12745168251995972No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG1211170.12703632888472807No Hit
GTCGCCTTGGTGCGCCTTTACCACACCAACTAGCTAATCCTACGCGGGCT1068300.11205108295908502No Hit
GTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCC1062670.11146056756354103No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG1056280.11079033783396267No Hit
GGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAA1030460.10808214822242698No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC994120.10427054440820518No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG990980.10394119834390533No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA981590.10295630676945454No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG965540.10127286589938685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)