FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31742081
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGA1220310.3844454936650184No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG799370.2518328902254392No Hit
AACTGGTCAACTATTCTAAAATCCTTAAGGGCCTTGGAAAAAAATGACAA728190.22940839953120906No Hit
AACTGGTAGCTCCCAGTCAAACTCCCTTTCTTATATGGGGCCATTATCCA666200.2098791191415585No Hit
GCTCCTACATCTGCTCTATTTCAGGTTCTGTGGATTCAGGATAATGGATA647890.20411075127683026No Hit
GCTCCTACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC645720.20342711619947035No Hit
CACACCTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT632050.199120530251309No Hit
CATGCCTCAGCTCGACTCGGTCCCCCTTTCTTATATGGGGGTACAGTGAA631420.19892205555143028No Hit
CTCCTAGTCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC631370.19890630359112246No Hit
GCTCCTAAATTCAACGCGTGCTTTATTTTCCACATATTCACTGTATTAGG623560.19644584739103907No Hit
CACACCTAGCAGATCGATAAATCCCCTTTCTTATATGGGAAGGAAGGCAG617400.19450520588111408No Hit
CTTACCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTT578280.18218087213626605No Hit
TTTATGCCAGAAAACAATGGTGATCCATTGAAGGATTTGGAGCAGTCAAG565200.1780601593197371No Hit
CTGCCTGCTCCTTCTCTTCTCTGTGGTGCTCCTCTGCTTCTTGAAATCAC564140.17772621776121106No Hit
CATGCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAG563950.1776663603120413No Hit
GCTCCTAAGATGCGACGTAATACTTCTTTCTTATATGGGGGGGTAACGGT561800.1769890260188045No Hit
CACACCTAGTCTCAACAAGTGTTTCCTTTCTTATATGGGGGCGGCGACGA560110.17645660976039979No Hit
CACACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT559410.17623608231609011No Hit
TTTATGCAGATCACGGGCTCTATTCCTTTCTTATATGGGGCTTCAGGAAC556950.17546108586894477No Hit
CTTACCGTCAAACCGTCTCGCGCCTCTTTCTTATATGGGGGGCCGATCGC552800.17415367316339467No Hit
CTGCCTAAACATTCTTCCCCCACCAACCCCTTCCTCTTAACCTGGGGAAC520980.16412912562349016No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACT519000.16350534799529998No Hit
CAGCATAGTGCTGTATCGCGCTGACGTTTCTTATATGGGGGCGGCGACGA499970.157510151902139No Hit
TTGCCGTCACTTACGACCTCCATGGGTTTCTTATATGGGGCGGGGAGGTA498150.15693678054693389No Hit
TCCCGATGTCCCCTTCCTTCCCGGCCATTCTCCCTCTTGTTTTCACTTCA471550.14855673766316707No Hit
TCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGATAACTTAAT469080.14777859082396014No Hit
CTGCCTACTCCACTTTTGCGAAGTGATGGATCACGCTGAGACCACATGAT466450.14695003771176818No Hit
CTCGTACTCGGATGTTGCACGTTCCCTTTCTTATATGGGGGGGAGGTAGT462790.14579699421723485No Hit
GCTCCTAAGATGCGACCCCGACCCCGTTTCTTATATGGGGGGGGGGATGC460720.1451448630604906No Hit
CTGCCTAAGCTCCTTCTTCCCCCGTCTTTCTTATATGGGGCCCCTTCTCC444970.1401829955635234No Hit
CTCGTACTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTC444300.13997191929539843No Hit
CTAATGGGTGCAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGG440690.13883462776117295No Hit
TGCCGTCCGATCTCGTTCTGATTAATGAAAACATTCTTGGCAAATGCTTT434340.13683412880207824No Hit
CGACCTTAGTACATGACTCGGGCCTCTTTCTTATATGGGGACGAAAAATA434340.13683412880207824No Hit
AGGCCGTTGGAAGCTGTGACTACAGTTATTTCTAAATGACTACCACATCT419900.13228496266517625No Hit
GATCGTATCCGCATCTACCAGGTCCATTTCTTATATGGGGGCATTCCGGT417610.13156352288307752No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC412250.12987491273807789No Hit
CTCCTAGTCTCATTCACGCTCCTTTCTTATATGGGGGGGGGCGGGCGCCG411910.12976779940798464No Hit
GCTCCTACCTCAGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACCA411770.1297236939191227No Hit
TCATTTGCAATGGATAGTGGTTCCCCTTTCTTATATGGGGGCCTTAGGGT401950.12663000891466442No Hit
CCTCAGTTCCTAGTGAGATCTAACCATTTCTTATATGGGGGTCGAGGCGG400050.12603143442296677No Hit
GCTCCTAAGATGCGACCGTCTGCTTATTTCTTATATGGGGCCGTTATGAA394940.12442158407950632No Hit
AACTGGTAGCTCCCAGCCCCAGACATTCAGGTAACCCCTTGCTATTCAAT394180.12418215428282728No Hit
CCTATTACAATACGCTCTCTGTGTCTTTCTTATATGGGGCTCTCGAGTCA391000.12318032960724912No Hit
AACCGCGAGGCTAGGTGGGCTGGGCGTTTCTTATATGGGGGTACAGTGAA389150.12259750707585933No Hit
ACCGTAAGTGCCTGTGTTACCGGCTCTTTCTTATATGGGGCCTCTTTCGG385410.12141926044483348No Hit
ACTGATGTCCCGGATGGCCCATGCTCTTTCTTATATGGGGCTCCCTTTCA384140.12101916065301453No Hit
AGGCCGTTCAATAAGGCCCACGTCCTTTTCTTATATGGGGCTCTTTCGGC383290.12075137732778139No Hit
TATCTCATCGCCCTTAACGGGGGACCTTTCTTATATGGGGGCGCTACCCT383120.12069782066273474No Hit
CATGCCTCAGCCTCCCGAGTAGCTGGGATTGCAGGTGCGCACCACCACGC381940.1203260743994699No Hit
AACTCTTAGGGCGTTGCGGCGCTGCAAGAAGCCGGAATCATCGACATCGC381430.12016540440433002No Hit
AACTGGTAGCTCCCAGACTGCAGACTTTTATATACACTTGAGTGTCTAAT380260.11979680853312674No Hit
ACCAGTAAGCGATCCCGCCCCGTCCGTTTCTTATATGGGGGACATGAACT378580.11926754266678356No Hit
AGGCCGTTCAATAAGGGCCAATCCCTTTTCTTATATGGGGGCCGACGCTC375720.11836653053717557No Hit
ACTGATGTCGGCTTGGCATTGCCTCGTTTCTTATATGGGCAAGTGATTTT374770.11806724329132673No Hit
GTACGTAGTAAACACAGTCATCGTACTTTCTTATATGGGCTTTGAAGTCA373560.11768604585187721No Hit
AGGCCGTTCAATAAGGATGTTATCTCTTTCTTATATGGGGCTCTTTCGGC371710.1171032233204874No Hit
TTGTAGGGTTACAGAATAGTTGTCCTTTTCTTATATGGGGGCCACACCCT369990.11656135588589797No Hit
CGAACATCAACCGCCAGTCCCTCGGCTTTCTTATATGGGGGTGCCCACCG364220.11474357966637411No Hit
TGGGAAGCAGTCGTGCGCTGTTTTCCTTTCTTATATGGGGGTCCCGGCCC363310.11445689398877157No Hit
AGATTGCGTTTAGCTGGATTTTCAGGTTTCTTATATGGGGTTTTCCTCAA354070.11154593172388413No Hit
GACACGCCACCCTATCCATAGTTCCCTTTCTTAAAATTATAAAGGAATGT352770.11113638075588049No Hit
CATGCCTTATCTCTTGGGTACATTTCAGAGCAAATTACAGATGTCAGTAC352280.11098201154486374No Hit
GGATTACCACCTCGTTGAATGTATCCTTTCTTATATGGGGACCCACGGCC352140.11093790605600182No Hit
ACCTTAAAGTTACATTCGTTCTTCCGCTCAAATCCTGATCTGGTCCATTA348810.10988882549950019No Hit
GTTCGGGCACCGCTAGGGTTGCTCGGTTTCTTATATGGGGACTCTTCTGG346380.10912328022853951No Hit
TATCAGGGTTCAACCACTGTGCCTCTTTTCTTATGGGATCCATTACTATT345170.10874208278908996No Hit
CAGCAGCGTCTACCTCCTTCAGCCTCTTTCTTATATGGGGGCCTTTGCCG343360.10817186182594644No Hit
AACTCTTCATTAACCGCGCTATTCCCTTTCTTATATGGGGCTCTTCCTGT343310.10815610986563862No Hit
AGGCCGTTCAATAAGGTCCGCCGTCTTTTCTTATATGGGCTTACAAAGAA343000.10805844771173007No Hit
ACCTTTACACTGAAGGACGCGCTCGGTTTCTTATATGGGGGGGGGGATGC342540.107913529676898No Hit
TATCAGGGTTCAACCATACCGCCCCCTTTCTTATATGGGGCCTTTTCCCA341590.1076142424310492No Hit
AACTCTTGGGCGATCCCAACCAGCGTTGCCGATCGTACGAGCCACGGCCT338730.10671323030144117No Hit
ATCTACTTCCCAACGGCACTTTTCCCTTTCTTATATGGGGGTCCCAGATG338100.10651475560156248No Hit
CACACTCAGATACACAGAGTGCTTCCTTTCTTATATGGGGGGCCTCTTGC337970.10647380050476211No Hit
CGCCAAGGTACCGAGACATCACCGCGTTTCTTATATGGGGCTCTCTCCTC336360.10596658738284992No Hit
GCAGCGGAGATGTACGGCTCCTCTTTTGACTTGGACTATGACTTTCAACG336250.10593193307017268No Hit
GTTCATTTCCGAATGTTCAGGCCACCTTTCTTATATGGGGGATGAAGTAC335030.10554758523866156No Hit
ACGGAGACAAGAAGAGATAGAGCCCTTTTCTTATATGGGGAACCAAAATG333150.10495531153108709No Hit
AGGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG332610.1047851903597625No Hit
GCTCCTCTGAGAATGGCACTGAGTATAGAACGGAAGTCACGTATTTGAAT332060.10461191879637634No Hit
TGAGAGGGTCTAAAGAGTGCCCCAGCTTTCTTATATGGGGAATAACAGCA331760.10451740703452934No Hit
TCATTACCAGCCACCACCTGCAGCCTTCAGATGACCATGGGCCCAGTCAA330450.10410470567446413No Hit
CACACAATCACGCGGTCCTCATACCGTTTCTTATATGGGGCGGCCAGCGC330210.10402909626498653No Hit
GCTCTAGAATAGTTTTATCTATACCTGTCAATGGTGCTTTTGTTCCCCAA328990.10364474843347542No Hit
CCACCTAGTTAAAGACACGATATCCTTTTCTTATATGGGGCTCTCTTCCA327070.10303987315765466No Hit
CAACTAGAGTGGTCCCCCAGCCTCATCTCCGGCTCCAGCCCCTAAGTTTT325360.10250115611512679No Hit
AACCAAGTGCAGACAATGGCCGTTTTTTCTTATATGGGGCCCTCTGCCTC325020.10239404278503353No Hit
TATCAGGGTTCAACCAATCGTCTCGCTTTCTTATATGGGGGGCGGCGGTG322160.10149303065542552No Hit
CAACCTCCATTACGACGCGTATTCTCTTTCTTATATGGGGCTTTTCGATC321690.1013449622285319No Hit
ACGGAGACAAGAAGAGTTCATTGGGTTTTCTTATATGGGGTTGTGGTTAT321600.1013166086999778No Hit
GCGCAACAGAAGCCCACCACGTCCTCTTTCTTATATGGGGCTGGTCGGCG320880.101089780471545No Hit
AACTGGTAGCTCCCAGCCTACGTTGTTCCTTTTAAACCAAACTTTAATAA320530.1009795167493902No Hit
TCTTCGGCAGCCTGTGCGATATCTTTTTCTTATATGGGGCTTCCGTCCTC319610.10068968067972607No Hit
AGGCCGTTCAATAAGGGGGGTACTATTTTCTTATATGGGGGTCCCAGATG319270.1005825673496328No Hit
TCCCATGTTCTGCCCACGCAGCCAGCTAGCAGTCAAGCTTTATTCCTATT319210.10056366499726342No Hit
CCTATTACAATACGCTGTTAATACTGTTTCTTATATGGGATAGTCTGTAA319040.10051010833221677No Hit
TCCCGATGTAGCGTCCATCGCCGCTCTTTCTTATATGGGGGGCCGCCATT319000.10049750676397053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCG545300.069.679552
AATACGC721700.069.608159
ATGTCGG695000.069.220825
ATTGCGT549400.069.146443
AACTGGT1390450.069.020061
CGTTTAG532850.068.736817
GCCGTTC1103100.068.283623
GATGTCG705550.068.270624
CCGAGCG240450.068.1914457
CAATAAG1069200.068.050299
TCCCGAT1646700.067.935771
ACTGATG775400.067.861981
ATAAGGG340150.067.8203911
GCGATGG242700.067.80376461
CGTTCAA1089250.067.7422565
CGCACGG119150.067.67090621
TCCGGAC131350.067.6226644
CCTATTA855300.067.449031
GTAGCTC1269350.067.146036
ACTGGTA1334450.067.104892
Produced by FastQC (version 0.11.5)