FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004724446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47973708
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC2790700.5817144674328697No Hit
GGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTG1576070.32852786780625753No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG1562410.3256804748134124No Hit
CTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGA1494940.31161652128286604No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT1468510.3061072535814826No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC1284070.2676611947527592No Hit
GCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACA1236480.25774117773010163No Hit
GCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGG1204430.25106043501994885No Hit
GATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGC1179100.2457804595800683No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG1147430.2391789269238892No Hit
GCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCC1062080.22138793190636835No Hit
GTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGA1054170.21973911209865202No Hit
GGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCC1048090.21847175123507234No Hit
GGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAA1010460.2106278714165684No Hit
GCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGC980790.20444323378130372No Hit
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG952020.19844619890545043No Hit
GGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCA935440.1949901391820703No Hit
CCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGA920710.19191970735303596No Hit
CCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACT904920.18862832116291697No Hit
CACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGG897970.18717961096523955No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC894700.18649798760604455No Hit
GCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAG880690.18357763798453938No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC865580.1804279961015313No Hit
AAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCA857760.1787979365697561No Hit
GTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAA850270.17723666471643176No Hit
GTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGC824330.17182953629517234No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT821350.17120836271400994No Hit
GGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGA817830.17047462747720063No Hit
CTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATC808080.16844226425024306No Hit
GCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCA789580.16458598530678514No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG766990.1598771560455573No Hit
ACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGC766030.1596770464355184No Hit
CACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCA756270.15764259873345624No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT755920.15756964210479624No Hit
GGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGT748660.15605631317887708No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA739900.1542303129872721No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA736870.15359871703058683No Hit
GGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAA726100.15135373734296292No Hit
ACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGG723030.1507138034858594No Hit
GCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCA709280.14784765021707305No Hit
CTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAA686790.14315966570689095No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA674140.14052280469960754No Hit
GCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAAC669140.1394805671473216No Hit
GTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGG667230.1390824324023484No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG656240.1367915942624239No Hit
GCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTC645160.1344819958465583No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT639480.13329801398716146Clontech SMART CDS Primer II A (100% over 26bp)
GGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAG634070.1321703129555881No Hit
CATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGG612830.12774288783347745No Hit
GGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTA611020.12736559783954995No Hit
CATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGA607220.12657349729981265No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC605810.126279586310068No Hit
GCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCG592400.12348430519483714No Hit
GCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAAC579860.12087037341370402No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT578610.12060981402563255No Hit
GACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTAGACCAC571950.11922155360598767No Hit
CAGGATCCACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAG568290.11845863571771438No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA568070.1184127772654138No Hit
GGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGC566420.11806883887315944No Hit
CAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCT560010.11673269033112889No Hit
GGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGAT559790.1166868318788283No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC559650.1166576492273643No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA556320.11596351901754186No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC548040.11423757363095637No Hit
GTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAG542760.11313697077574242No Hit
GCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATC540710.11270965337930518No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC538600.11226982913224051No Hit
GCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGG532080.11091075136405966No Hit
CAAAGAACCTCTGGGTCCAAGGGTAGACCACCAGCAGCCTGCCCAGGGCC529810.11043757551532185No Hit
GAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCAT526600.10976845900675428No Hit
AGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCA526020.10964755945068912No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT521870.1087825022822918No Hit
AGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGC517010.10776944738146987No Hit
GCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGA515520.10745886059088866No Hit
GCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCA515290.1074109176634835No Hit
GCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTC514000.10714202037499373No Hit
GCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCC506280.10553280559426426No Hit
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC503630.10498041969155272No Hit
CCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCA502520.10474904295494523No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT501810.10460104522252063No Hit
CCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAG501740.10458645389678863No Hit
CGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCA501100.10445304749009603No Hit
GCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGT486920.10149726179181315No Hit
CAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCA485200.10113873207382676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)