FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004724449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136419959
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGACG11618470.8516693660639497No Hit
AACCGCGGGAACCCCGCATCTTCACGGG10798070.7915315382846582No Hit
GACGGCTCTATGAAATCTTATCTCGAGG6266940.4593858586337795No Hit
CGCTTCACCAACTAAGAACGGCCATGCA5310520.3892773490717733No Hit
ACGCCGAGTTCCTTTTACTTTTTTTAAC4945880.3625481224488566No Hit
GTGGGCAAATTTAAAGTTGAACTAAGAT4313770.3162125272299781No Hit
CCACTACCCATTACCAGATCGCTTCACA4104030.30083794410171316No Hit
CTCCTAGATTCAAGCGATGCAGTCTTAA3711790.2720855531117701No Hit
CCACGACCCATTACCAGATCGCTTCACA3538100.25935354518029136No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA3208100.2351635364441064No Hit
CCTGGATTCAAGCGATGCAGTCTTAAAG3058500.2241973991503692No Hit
CTCCTGGATTCAAGCGATGCAGTCTTAA3039780.2228251659275165No Hit
CCACCAACTAAGAACGGCCATGCACCAC2870980.21045160994367398No Hit
TCCACACCCATTACCAGATCGCTTCACA2768380.20293071631842374No Hit
GACGGCTTTTGGAGATTAGCTCACCCTT2765850.20274525958477968No Hit
CATGCCTGCATTCGCACTTCCGATACCT2505890.183689396945208No Hit
CTCTACGACCCATTACCAGATCGCTTCA2487900.18237067495380202No Hit
TGACGGCTTTTGGAGATTAGCTCACCCT2413140.1768905384292045No Hit
AGTTGGTTGATTGTAGATATTGGGCTGT2088770.15311322590267015No Hit
CCACCTACTAAGAACGGCCATGCACCAC2074550.1520708564353109No Hit
CCTCCACGACCCATTACCAGATCGCTTC1978250.14501177206775148No Hit
TGACGGCTCTATGAAATCTTATCTCGAG1965280.14406103142136262No Hit
GGGCATCATTTCAGAGCTTGCACCCCTC1634080.11978305901704603No Hit
AGCTCTCGAGTTGCAGAGAACAATCCGA1617110.1185391061435519No Hit
AGGCCGTACTCCCCAGGCGGATAACTTA1616900.11852371250162888No Hit
CTCGAGTTGCAGAGAACAATCCGAACTG1602590.11747474575915977No Hit
ACGCAGGCTTATGCGGAGGAGAATGTTT1579540.11578511030046564No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATT1502880.11016569796799308No Hit
ACGCCGACTGCCGGCGACGGCCGGGTAT1496880.10972587962733518No Hit
ACTGTCCTTTGAAGTATACTTGAGGAGG1486910.10899504815127528No Hit
CGTCCATACATAGCTACCCTGCTGCGCT1458320.10689931375804035No Hit
ACACCCTTGCGAGATTGCTGCCCACTGT1443500.1058129624566153No Hit
GTACTCCCCAGGCGGATAACTTAATGCG1421750.10421862097173039No Hit
CGTTCATTATCGGAATTAACCAGACAAA1369090.10035848200188946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)