FastQCFastQC Report
Thu 29 Sep 2022
EGAF00004724450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136419959
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13209360.9682864660588264Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6862010.5030063086296632No Hit
GTCGCCTTGGTGCGCCTTTACCACACCAACTAGCTAATCCTACGCGGGCT2619720.19203348389805627No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2116840.1551708427063814Clontech SMART CDS Primer II A (100% over 24bp)
GGCTGATCATCCTCTCAGACCAGCTAAGGATCGTCGCCTTGGTGCGCCTT1981100.14522068577956398No Hit
GCCTTGGTGCGCCTTTACCACACCAACTAGCTAATCCTACGCGGGCTCAT1829680.13412113692249386No Hit
GGATCGTCGCCTTGGTGCGCCTTTACCACACCAACTAGCTAATCCTACGC1481090.1085684243608371No Hit
GGGTGGTCCCCCTATGTTCAGACAGGATTACACGTGTCCCGCCCTACTCA1407790.10319531029913298No Hit
GTCCCCCTATGTTCAGACAGGATTACACGTGTCCCGCCCTACTCAAGTCC1404160.10292922020303495No Hit
GTTCAGACAGGATTACACGTGTCCCGCCCTACTCAAGTCCGACAATTTCA1391170.10197701349551058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)