FastQCFastQC Report
Wed 11 May 2022
EGAF00004724451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131967358
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGACG10681950.8094388007676869No Hit
AACCGCGGGAACCCCGCATCTTCACGGG10414380.7891633323446544No Hit
GACGGCTCTATGAAATCTTATCTCGAGG6038990.4576124044250397No Hit
CGCTTCACCAACTAAGAACGGCCATGCA5134940.3891068274625912No Hit
ACGCCGAGTTCCTTTTACTTTTTTTAAC4760730.3607505728803027No Hit
GTGGGCAAATTTAAAGTTGAACTAAGAT4162750.31543785244226835No Hit
CCACTACCCATTACCAGATCGCTTCACA3982570.30178447612780124No Hit
CTCCTAGATTCAAGCGATGCAGTCTTAA3577930.2711223483007063No Hit
CCACGACCCATTACCAGATCGCTTCACA3442820.26088421047271404No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA3104370.23523771689056622No Hit
CCTGGATTCAAGCGATGCAGTCTTAAAG2954530.2238833939526167No Hit
CTCCTGGATTCAAGCGATGCAGTCTTAA2944180.22309910909938802No Hit
CCACCAACTAAGAACGGCCATGCACCAC2783940.21095671249249376No Hit
TCCACACCCATTACCAGATCGCTTCACA2707400.205156793394318No Hit
GACGGCTTTTGGAGATTAGCTCACCCTT2657080.20134372925765476No Hit
CATGCCTGCATTCGCACTTCCGATACCT2415210.18301571211268775No Hit
CTCTACGACCCATTACCAGATCGCTTCA2399800.18184799910899177No Hit
TGACGGCTTTTGGAGATTAGCTCACCCT2346870.17783715879194914No Hit
AGTTGGTTGATTGTAGATATTGGGCTGT2018050.15292039111671843No Hit
CCACCTACTAAGAACGGCCATGCACCAC2011970.15245967112564304No Hit
CCTCCACGACCCATTACCAGATCGCTTC1918390.1453685236314271No Hit
TGACGGCTCTATGAAATCTTATCTCGAG1900870.14404092260451254No Hit
GGGCATCATTTCAGAGCTTGCACCCCTC1574690.11932420439909087No Hit
AGGCCGTACTCCCCAGGCGGATAACTTA1570290.11899078861607582No Hit
AGCTCTCGAGTTGCAGAGAACAATCCGA1570100.11897639111635469No Hit
CTCGAGTTGCAGAGAACAATCCGAACTG1554220.11777306324492758No Hit
ACGCAGGCTTATGCGGAGGAGAATGTTT1520880.11524668092544521No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATT1468190.11125402692383976No Hit
ACGCCGACTGCCGGCGACGGCCGGGTAT1450560.10991809050234984No Hit
ACTGTCCTTTGAAGTATACTTGAGGAGG1431810.10849728460881972No Hit
CGTCCATACATAGCTACCCTGCTGCGCT1403560.10635660372923432No Hit
ACACCCTTGCGAGATTGCTGCCCACTGT1388040.10518055533096297No Hit
GTACTCCCCAGGCGGATAACTTAATGCG1376890.10433564942627707No Hit
CGTTCATTATCGGAATTAACCAGACAAA1324580.10037179042411383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)