FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100258815
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACGCTCTTCCGATCTACACGACG9245670.9221802591622492No Hit
CCACCTACTAAGAACGGCCATGCACCAC2581470.2574806015810181No Hit
CGTTCGTTATCGGAATTAACCAGACAAA2346790.23407318349014994No Hit
CCACCAACTAAGAACGGCCATGCACCAC2107180.21017403806338625No Hit
GTGGGTCGCGTAACTAGTTAGCATGCCA1867540.18627190038102884No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1809480.18048088838871673No Hit
CGTCCATTCTCGACTGCCGGCGACGGCC1652220.1647954845666189No Hit
ACGGCCATGCACCACCACCCACGGAATC1590630.15865238383278318No Hit
GACGGCTCTATGAAATCTTATCTCGAGG1358110.1354604081446604No Hit
AACCGCGGGAACCCCGCATCTTCACGGG1322840.13194251298501783No Hit
GTGGGGTCGCGTAACTAGTTAGCATGCC1111900.11090296648728594No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1032170.10295054853780188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACA1211100.020.91649617
GATCTAC1213700.020.91065816
CGATCTA1277050.020.23855615
TGTCCCA1295950.020.1292651
ATGCGAT440850.020.052211
CCGGTAG433750.019.8375721
GGGCACC542500.019.7743155
CGACGCT1375500.019.6840524
TGCTACC799200.019.6756171
CCACCTA827150.019.6532781
CGTTGGG719500.019.593291
ACGACGC1393050.019.5150153
TGGGCAC529300.019.509024
TGCGGGT785950.019.4949251
GACGCTC1391250.019.4729375
CAACGAC340950.019.4612852
GTCCCAA546600.019.4276642
GCAGTTA466600.019.4101581
TTGGGCA532600.019.3963833
GTGGGTC884550.019.3643861
Produced by FastQC (version 0.11.5)