FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49034395
Sequences flagged as poor quality0
Sequence length28
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTAAGTTTCAGCTTTGCAACCATA2326100.4743812990860803No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT2130740.43453987756961215No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1099580.22424667419675515No Hit
TGCGCAGGTCCAAGAATTTCACCTCTAG1075920.2194214897522443No Hit
CCTAGCTGCGGTATCCAGGCGGCTCGGG922330.18809857855898907No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA626940.12785719085552089No Hit
GGGCATCGTTTATGGTCGGAACTACGAC624610.12738201419636155No Hit
GGGCATCTTCTCGCGGTTGGCCTTGGGG573680.11699542739336338No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGT494070.10075988497461832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA645500.021.6785761
TAGTGCA490850.021.3602036
ACACCGA699150.021.3537339
AGATCTG934400.021.2967911
CACCGAC701250.021.28351210
GGGCATC1018200.021.2473551
AACGCGA486300.021.24557110
ACACTGA523300.021.1996841
CACCTAG420350.021.1846623
AACAAGT370450.021.178616
CACACCG1176750.021.168738
GCCGAGA229450.021.1621594
AAGTTAA370100.021.1517459
GGGAGAT513500.021.14781
AGCATAC401050.021.1285021
GAATAAG278400.021.08041
GCGCAAC266950.021.077661
CCTTTAG504350.021.0515082
ACGGGCA495850.021.0506972
CGTCATA520850.021.0428877
Produced by FastQC (version 0.11.5)