FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49034395
Sequences flagged as poor quality0
Sequence length89
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACG3998920.8155336677448555No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC3294210.6718161812743891No Hit
GGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGT2932350.5980190027836583No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2687640.5481132172631069No Hit
GCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAAC2633080.5369863337765257No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT2461290.5019517422413389No Hit
GGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGC2384360.48626275494986737No Hit
GCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCG2383620.4861118404744262No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC2296060.4682549871370902No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC2261380.46118240063938787No Hit
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC2122010.4327594946363669No Hit
GCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGT2101840.4286460554881935No Hit
GCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTC2059950.4201030725473415No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT2015200.4109768255527574No Hit
CTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTT1731610.3531419119171349No Hit
GACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACA1711020.3489428186072246No Hit
GCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGA1703150.3473378227670597No Hit
GTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGG1663780.33930876479662897No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC1619730.330325274738273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1553670.3168530987279439No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT1522230.3104412729064976No Hit
GGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGC1495050.3048982250112396No Hit
GACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGC1492980.30447607235696494No Hit
GGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGC1444580.2946054499091913No Hit
GTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAG1437530.2931676836228937No Hit
GCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCG1436640.29298617837540364No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC1422650.2901330790356443No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG1406610.2868619058112168No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC1386550.28277089989587917No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG1248280.2545723262212167No Hit
GCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGG1213800.24754052741957966No Hit
ACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAG1209540.2466717494933913No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC1200780.24488524840573642No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC1180890.24082891203205423No Hit
CCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGG1170580.23872630629989416No Hit
AGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTG1166460.2378860797609515No Hit
CAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGT1155040.23555710231562965No Hit
GCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACG1152130.2349636413378813No Hit
AGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCG1139820.23245315864506944No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG1137250.23192903675063187No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC1102610.22486460779214262No Hit
CCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTG1086060.22148942594274898No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC1061130.21640523962822422No Hit
GGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCG1025850.20921029004232639No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA1025080.20905325741247546No Hit
CAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGG1023470.20872491645915078No Hit
CCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAG1020720.20816408563825453No Hit
GCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGG1011220.20622667007515844No Hit
CCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCG994440.20280458237528984No Hit
GGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCA967120.19723298309278617No Hit
GTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGG963700.19653551349007162No Hit
GTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGT956730.19511406228220007No Hit
CTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCG955750.19491420257148068No Hit
GCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGG947300.19319092241272684No Hit
GTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGT937850.19126370377364704No Hit
GCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGC936180.19092312651150278No Hit
GGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAG933500.1903765713842294No Hit
GCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAG930450.18975455901923538No Hit
GCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCC922980.18823113857120088No Hit
CGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCC914990.18660167011339693No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA905590.18468464839833346No Hit
GGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGG900320.18360989260701596No Hit
GTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCA898270.18319181872234785No Hit
TGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGG886840.18086080189222278No Hit
TGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGAC884180.1803183255345559No Hit
CCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACC881120.17969427378475863No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT878030.1790641038805516No Hit
ATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTT868640.17714912155029136No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT867840.17698597076603065No Hit
GGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTG866440.1767004568935744No Hit
GTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCG852480.17385347570822482No Hit
GGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACC840130.17133483547619993No Hit
GAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGG828910.1690466457269433No Hit
GAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAA789450.16099923329328322No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG785740.16024262153127414No Hit
CAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCA780760.15922700789925112No Hit
TGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTG780020.15907609342380996No Hit
GCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCAC778940.158855839865058No Hit
GCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCT761660.1553317829250264No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA756880.1543569569890686No Hit
GTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCT755410.15405716742298953No Hit
CGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCA749180.15278663069055914No Hit
CACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGG747710.15248684112448008No Hit
CAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGT734940.1498825467307183No Hit
GTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCA730590.14899541434130062No Hit
AGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTC727870.14844070167481418No Hit
ACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCC727110.1482857084297665No Hit
ACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGG713850.14558148418064504No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC708010.14439048345554176No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC692320.14119068869922838No Hit
GCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTA689560.14062781849352887No Hit
GGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCG688460.1404034861651704No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA680460.13877197832256316No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG673180.1372873061857906No Hit
AAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGC669560.13654904888701086No Hit
CCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGC664190.13545389924766077No Hit
CGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTC657750.13414053543436194No Hit
TGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTT657540.1340977083534935No Hit
CGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCAT648090.13217048971441372No Hit
GGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGG647480.13204608724141492No Hit
AGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTC641620.13085100774670513No Hit
ACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGT635980.12970079471766707No Hit
CACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCT632600.1290114826541655No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG600700.12250584513176924No Hit
CGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAA598890.12213671648237936No Hit
GAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATG589260.12017278891684091No Hit
ACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTG586780.11966702148563269No Hit
CCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTG581670.11862489585116733No Hit
AGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTC568710.11598185314614365No Hit
AACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGG566120.11545365248209956No Hit
CATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCT549570.11207847063270587No Hit
CTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGT542160.11056728649349096No Hit
TGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGG532760.10865026477842746No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG527130.10750209113419265No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC525910.10725328618819503No Hit
GTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCG524270.10691882708046058No Hit
TAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTG523840.10683113353392043No Hit
CACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGC522710.10660068305115215No Hit
ACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAA503490.10268098545928833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT434100.063.055521
GTATCAA434150.063.000379
AGTGGTA453300.060.3475955
TGGTATC461500.059.1769037
GTGGTAT461850.059.1676376
GGTATCA464200.058.9309848
CAGTGGT494800.055.493914
AGCAGTG503550.054.5813332
ATCAACG437200.032.59509310-11
CAACGCA444800.032.126412-13
ACGCAGA444300.032.0689414-15
AGAGTAC445850.031.50491118-19
TCAACGC446400.030.73767912-13
AACGCAG456750.029.99553314-15
GTACTTT418850.029.7020922-23
CAGAGTA456500.029.60183518-19
CGCAGAG476400.028.75777216-17
CTACACG26950.028.5431561
TACTTTT462900.027.96479822-23
GAGTACT452800.027.85996820-21
Produced by FastQC (version 0.11.5)