FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51033928
Sequences flagged as poor quality0
Sequence length28
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTAAGTTTCAGCTTTGCAACCATA2420250.47424333082885567No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT2245210.43994457961378164No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1142720.2239137853547154No Hit
TGCGCAGGTCCAAGAATTTCACCTCTAG1125700.22057874910196995No Hit
CCTAGCTGCGGTATCCAGGCGGCTCGGG967510.18958172296672912No Hit
GGGCATCGTTTATGGTCGGAACTACGAC655660.12847531548032126No Hit
ACGGGCTTTTTAACTGCAGCAACTTTAA651490.12765821200359104No Hit
GGGCATCTTCTCGCGGTTGGCCTTGGGG597630.11710444863268217No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGT524920.10285706403003116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA656950.021.680791
ACACCGA741350.021.3753329
CACCGAC742450.021.340710
AGATCTG981600.021.2842921
TAGTGCA504450.021.275436
GGGCATC1057600.021.2366031
AACGCGA508900.021.20092810
AACAAGT375000.021.1948976
CACACCG1234550.021.187718
ACACTGA545400.021.1733991
AGCATAC409800.021.1675451
GGGAGAT539300.021.1659681
GAATAAG286200.021.1571431
AAGTTAA376300.021.1098849
GCCGAGA238650.021.102754
TACTTTA429350.021.0555022
CACCTAG432900.021.0486773
TACATTA452000.021.042292
GCGCAAC274950.021.0301511
TCCGCTG606200.021.0295568
Produced by FastQC (version 0.11.5)