FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32333776
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2987010.9238048782177497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1041150.3220007462165879No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA532160.16458331374597263No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT511080.15806381537374417No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC499850.1545906670473625No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT491000.1518535911178453No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA486460.1504494866297088No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC448110.13858882426846775No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT446320.13803522360023773No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA428540.132536329811897No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG413810.12798072207836164No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG401890.12429417461171254No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT391660.12113030040166047No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA385770.11930867585647899No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT375300.11607057585850784No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT344170.10644287261716665No Hit
GCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG332600.1028645710912329No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA330840.10232024864649275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT417750.067.522951
GTATCAA488400.057.761679
GGTATCA494750.056.9782838
TGGTATC497100.056.7174457
GTGGTAT504950.055.8274086
CAGTGGT521800.054.293184
AGTGGTA533450.052.9764565
GCAGTGG562050.050.5517353
AGCAGTG570050.049.7809332
AGAGTAC491300.030.3343818-19
ACGCAGA499250.030.2807714-15
CAACGCA504350.030.00348512-13
ATCAACG504350.029.92166710-11
GCAGAGT527850.028.46232816-17
AGTACTT496750.028.3020520-21
TCAACGC505500.028.03066812-13
GTACTTT472300.027.8079422-23
AACGCAG512600.027.64635814-15
CGCAGAG513400.027.56201216-17
CAGAGTA513700.027.3152918-19
Produced by FastQC (version 0.11.5)