FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33422181
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTTAAGTTTCAGCTTTGCAACCATA1210230.3621038375682305No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1176480.35200575330496836No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG857180.25647039611209094No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA855780.256051512616726No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC844540.25268847655393883No Hit
CAGTCCTTTGAAGTATACTTGAGGAGGG586660.17553013670771517No Hit
TAGTTGGTTGATTGTAGATATTGGGCTG519020.1552920798316543No Hit
CCACCTACTAAGAACGGCCATGCACCAC503000.15049885583469255No Hit
AGGTCCGTCTTGCGCCGGTCCAAGAATT487880.14597491408475108No Hit
TGCCCTACAATGGATCCTCGTTAAAGGA476360.14252810132289093No Hit
AGGCCGTCCCTCTTAATCATGGCCTCAG467070.1397485101286478No Hit
CATGCCTGTGTTGGGTTGACAGTGAGGG457740.1369569508345371No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG414900.12413911587636965No Hit
CCCTCCTAATCATGGCCTCAGTTCCGAA405850.12143133328133193No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT403730.12079702398835072No Hit
GTGCGGTGTGTACAAAGGGCAGGGACTT393220.11765240574814673No Hit
ATTGGTGGCTGCTTTTAGGCCTACTATG383680.11479801392973127No Hit
AGGGTGATAGATTGGTCCAATTGGGTGT380760.11392434263939866No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTA361560.10817965470296509No Hit
CCTAGCTGCGGTATCCAGGCGGCTCGGG355550.10638144769786269No Hit
ACGCCGAGTTCCTTTTACTTTTTTTAAC352010.10532227085958273No Hit
TTGGCAAATGCTTTCGCTCTGGTCCGTC351250.10509487696209892No Hit
TACGGGCGGTGTGTACAAAGGGCAGGGA350140.10476276219077384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAA225000.020.8259287
TATCATA231500.020.4290286
TCATAAA237950.020.36218
TAGTTGG291550.020.2547451
TACCTTA236450.020.232031
CCCTACA181150.020.186173
GCCCTAC187550.020.0816042
TTTATCA238800.019.979584
TGCCCAT303450.019.9319021
CAATGTA148050.019.925668
CTTTATC252800.019.9131583
AATGTAA148300.019.869829
CCCTCCT405400.019.8355641
GGATCGA94250.019.82779310
TGCCCTA245100.019.8098561
CAGTCCT498400.019.7599411
TACAATG145100.019.7577846
AGGCCGT301150.019.6711921
CTGCCTA165050.019.5895941
CATAAAG246800.019.5695429
Produced by FastQC (version 0.11.5)