FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33422181
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3105520.9291793375183984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1146410.3430087342295226No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA542500.16231735445391787No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT526110.15741342553318108No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC514520.15394566859655268No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT504680.15100151602913048No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA497650.14889812247740503No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT460830.13788148654930688No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC459250.13740874660453786No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA443240.13261851463254298No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG422440.12639510270140658No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG417460.12490507426789411No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT408870.12233492482133347No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA394730.11810420151814748No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT383030.11460353230688326No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT353750.10584288320382204No Hit
GCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG335930.1005110947128196No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA334330.1000323707181168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT446750.067.979991
GTATCAA520550.058.3075949
GGTATCA529750.057.287078
TGGTATC532300.057.0048337
GTGGTAT540650.056.132366
CAGTGGT553050.054.826984
AGTGGTA572150.053.056395
GCAGTGG594650.051.1867643
AGCAGTG607100.050.1513942
ACGCAGA530050.030.66654414-15
AGAGTAC523850.030.52896318-19
CAACGCA534050.030.50290312-13
ATCAACG538550.030.28324510-11
GCAGAGT562750.028.6625916-17
AGTACTT536650.028.55621720-21
TCAACGC537450.028.32556212-13
AACGCAG543200.028.01044514-15
GTACTTT509850.027.98588822-23
CGCAGAG545850.027.85090316-17
CAGAGTA543150.027.62586818-19
Produced by FastQC (version 0.11.5)