FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004724465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24037055
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACCAAGTAATGGTCCTTTTTCGT342449714.246741125316726No Hit
CGTAGGACTTTAATCGTTGAACAAACGA1855110.7717709178599458No Hit
CGGACGTTCAAAGTAGGCTCAGTCCATT1817280.7560327169863363No Hit
TGCACCTGAAACTCTGGACGTGCTGAAT1814040.7546847981169075No Hit
TGCGTGGGTATAATACTAAGTTGAGATG1683160.700235532181459No Hit
TTTGCGCTGTTATCCCTAGGGTAACTTG1544460.6425329558883149No Hit
GCAGGCAATCCCCTTTGTTGGCCATCCC1394030.5799504140586274No Hit
GAACATCTCAACGCTAGCAGTGTTATTT1348630.5610629089129263No Hit
GGACAAGTATGTCCATGTTTTCCACCAC1311400.5455743226447666No Hit
GGACAAGGTACCATCAACTCCTCTAGTT1124840.4679608213235773No Hit
AGGCCACCACAACGCCTCCACATCTTTT1096980.4563703831438585No Hit
GCACTCTGGTACAGCTTGGTGATGATGG1046240.43526130800965424No Hit
GAACATCTGGAGGCACCGTGGCCGCTTT982990.4089477683518218No Hit
GAACATCTCAACGCTAATGTGTGTTTTT965910.401842072583351No Hit
AACCATGTCGCCTGAGAACAATGCTTTT860830.3581262346822437No Hit
GAACATCTCAACGCTACCTGCAGGTGTT781060.3249399728876936No Hit
CTCTACGTCCACGTTCAAAACTCGGGTT769790.32025137854866165No Hit
CATTCGCTCCTATGTTCCCTCACTTTTT763830.31777187346786034No Hit
GCACTCTCAAGTCATCTAGTACTTCATT752570.3130874393722526No Hit
GACATCTCAACGCTACTATTCTGGGTTT736320.3063270438079873No Hit
CTAACTTGTCGATTGTACTTACAGGTTT720500.29974553871096105No Hit
GCGCAGTTATCCCTAGGGTAACTTGTTC719760.299437680697573No Hit
AGGTCATTCGTCTGAAACGGACTGCCTT715430.2976362952949103No Hit
TGCGCAGAGGGAAACATTCACTCTGGTT695480.28933660966370467No Hit
TGGTTAGCACGACTCGACCTGAGGTATT693990.28871673339350434No Hit
CATTCGCTCCTATGTTCGTCTCACGCTT688360.2863745163457004No Hit
GCACTTACCAGCACACTCTTCAAAGCCC688060.28624970904297553No Hit
GCACTCTACTCTCAGTTTACTGCTAAAT684340.2847020984891868No Hit
ATCTGCCAGATTACCCCTCTTTGTGGTT673010.2799885426896099No Hit
TGCGGGTACACTGCATCTTCACAGCGAG670240.27883615526111666No Hit
TGACAACTCTGCTTGCCCAACTGCGTTT664660.2765147394304336No Hit
GCACTCTCAAGTCATCCTCTGACAGCTT648940.26997483676764894No Hit
GAACATCTCAACGCTACGTTCTACGCTT644190.2679987211411714No Hit
GACATCTCAACGCTAACGGTAATTGTTT641250.26677560957446744No Hit
CGTAGCGAGGACATTACTGTGAATCATT637380.2651655953693162No Hit
GCAATCACTTTTCCTCTTGGCCCATTCG634970.2641629767040929No Hit
CGTAGCGTGCTAAAATAGCAATATCATC617830.25703231947507715No Hit
CAGATCACGTAGGACTTTAATCGTTGAA616020.2562793154153036No Hit
AGCGTATCAAGGTTCTTTACGCATAGTT611440.2543739239270368No Hit
GCAATCACAGGAATGCAGCACCTACATT590910.2458329441772297No Hit
GCACTCTCAAGTCATCAATTTTCCACTT590300.24557916932835575No Hit
GCACTCTCCCACAGCCTTGGATGTGGCA583290.2426628386880173No Hit
CATTCGCTCCTATGTTCCCTATTCTATT572200.23804912873062029No Hit
CCTACACATCAAGGCTCAATGTTCAGTG569300.23684265813761293No Hit
CGTAGGCCAACACGCCTCCCGATCTGTT557770.23204589746955276No Hit
GCACTCTCCTTTGATTTTTGGGCCCTTC553870.23042340253412907No Hit
CGTAGCGGCTGCACCATCGGGATGTCCT548190.22806038426920439No Hit
GACGCGTAGAAACGAGGCAAATTACCTT545170.22680399075510707No Hit
GCACTCTTTTCTTTGTGCACTTGATGGA544790.22664590150498887No Hit
GCACTCTAGAGTAATCTGTCGTTGCGTT527510.2194570008680348No Hit
GCACTCTCAAGTCATCACGTTCGAAGTT521800.2170815018728376No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA518980.21590831322722356No Hit
ATCTGCCAGATTACCCCAGCCGTTCATT518760.21581678787189196No Hit
GCACTCTCAAGTCATCATAGGAGTACTT506210.2105956823745671No Hit
GCACTCTTCTGGGCTAAAGGTGAAGTCA505230.21018797851899912No Hit
CCTACACGTACACCGCGTCTAGGACTTT504750.20998828683463927No Hit
ATTGGACTCTAGAATAGGATTGCGCTGT504430.20985515904506602No Hit
CCGGGATCATCCGGGTGAACTTAGATTT499780.20792064585283013No Hit
GCACTCTCAAGTCATCCAGGAAAGATTT497470.2069596296218484No Hit
GCACTCTGCTGCAGCCTGGCTGAAGGAA489770.20375624218524271No Hit
CAGCACGAAACTCCCCCATTATATTAGA473410.1969500839433117No Hit
CCTAGCTGTAGCAAATGGCAACTATATT473330.19691680199591838No Hit
GCACTCTCAAGTCATCCGCGATACATTT470850.1958850616267259No Hit
GCACTCTCAAGTCATCACCTGTCATCTT463190.19269831516381686No Hit
GACGTGCGTGGTCCGTTCTCAAGCGTTT455500.18949908797063533No Hit
AGCGGTCGTTGGAGGTGTGATTCCAATT451840.1879764388773916No Hit
CGTAGCGAGGACATTAGACCCTACTATT435770.18129092769476127No Hit
TGGCTGGCAATAACGAACCATCAGAATT433730.18044223803623197No Hit
CACCAGGCACATCTTTGCGCACCGACTT430380.17904855648913728No Hit
GCGCAGTTGGGCAGCGGCCGTGAGAACA428280.178174905370063No Hit
CTGTGCTCAGTTTACGTGTCTAGAAGTT425900.17718476743511216No Hit
GAACATCCTTGGCTTGAAAAGCAATAGT425740.17711820354032554No Hit
GAACATCTCAACGCTAGCTGCTCGGGTT422500.17577028467089667No Hit
AGGTCCGTCCACGCAGATCTCAGCTGTT420810.17506720353221308No Hit
TGGTGGAGACAGCCTGGCCATCATTACG418380.1740562643801414No Hit
CGATTCGTCTCACCTTGGTGGTGTCTTT417480.17368184247196672No Hit
CGTAGCGACGGGCGGTGTGTACAAAGGG412200.17148523394400855No Hit
GCACTCTACACACTCTTGAAACTCCTTG411600.17123561933855874No Hit
GCGCAGTTCTAATTGTGGTATCATACAC410800.17090279986462567No Hit
GCACTCTCAAGTCATCAGTTGCGTGATT407900.16969632927161835No Hit
TGCCCATTCACCTTATCAAGCTCCTATT407330.16945919539644103No Hit
CAGCAGCTTCCTTCACCAAATCGCACTG403910.16803639214537722No Hit
GCACTCTAGAATAGGATTGCGCTGTTAT402790.16757044488187092No Hit
CACTCTCAAGTCATCACTGGACAGTTTT402710.16753716293447762No Hit
ATCTGCCAGATTACCCCGCCTCTTCTTT400880.16677583838785576No Hit
GCGCAGTAGGTGATTACTGGGTGAGATT399190.16607275724917217No Hit
GCGCAGTAGGTGATTACACCTATAGGTT397160.16522822783406704No Hit
ACCGTAACATTTCAGGTTTAAGTGATTT391820.16300665784556384No Hit
GCACTCTCAAGTCATCTTATTGGTTATT390900.16262391545054086No Hit
GGATTACTCCGGTCTGAACTCAGATCAC387030.16101390124538967No Hit
GAAACTCACATGACTTGCGACCACGTTT385710.1604647491134001No Hit
CCTGCTGTCATTGCTACTAAGGATTCAT385370.16032330083697857No Hit
ACTGCTCGGAGGTTGGGTTCTGCTCCGA381920.15888801685564224No Hit
CGACCTTTCGTACAGGGAGGAATTTGAA381830.15885057466482477No Hit
GCAAACTCACATGACTCCTCAACCGATT381490.15870912638840323No Hit
CAGCAGCCAGGAATCGTAGTCTGGCCTT381240.15860512030279916No Hit
TCAATCTAGCCCTAATCTACCTATAGTT377790.15716983632146284No Hit
GACTGGTGAAGTCTTAGCATGTACTGCT377620.15709911218325207No Hit
CAAGGCCGTTCGCTAAACCATATTTGTT376760.156741331248774No Hit
GCACTCTCCAGTGGTGGGATCACAGTCC374060.15561806552424995No Hit
GACTGCGCCGAGGGGATAGTCGAGATTT373510.15538925213592097No Hit
GGACAGAAATTGAAGGATGAGAGTAGCC372270.15487338195132475No Hit
TGCGTGGGTGTGGGTATAATACTAAGTT370560.1541619803257928No Hit
CTGTGCTAGAGTAGTGTTTGTTCTGTGG370050.1539498079111605No Hit
TGCCAGCTTCCTTCACCAAATCGCACTG363060.1510417977576704No Hit
TCGCGTTGTGAAGTAGGCCTTATTTCTC361780.15050928659937748No Hit
GCACTCTGAATGCTACCAAAGCTTTGTC361260.150292953941321No Hit
TGGGCGTCACCTCGTTCTTATTCTTGTT359490.14955659085524414No Hit
GACTGCGAGACTAGATCCCGTACTGGTT359230.14944842452621587No Hit
GCACTCTCAAGTCATCCCGGGATAGATT356110.14815042857787694No Hit
GCACTCTCGGGATGTCCTGATCCAACAT354440.1474556679260417No Hit
CATCCACAGTCGCCGTCTACACTCGTTT353980.1472642967285302No Hit
CGTAGCGTGCCAAGCAGAAATCACTTCA352200.1465237733990291No Hit
GGACAAGTTATTGGATCAATTGAGTATA347660.144635022884459No Hit
GTGTGCGCAATTGCTGCTTTGCCATGTT347350.14450605533830996No Hit
CCATTCGTACAGGGAGGAATTTGAAGTA345110.1435741608112974No Hit
GACGCGTAGAAACGAGACCTAACATTTT343030.14270883017907143No Hit
CGTTAGAAGGTGCAACACATAGTTTTTT340590.1416937307835756No Hit
GGCCGATTCCGATATGTATGATGGTCTT340030.14146075715182246No Hit
GGATGTTGTGGTCCGTTCGCAAATTATT338520.14083256039477382No Hit
AACCATGTGTCGGTTTGGGGTACGATTT329870.13723394983287263No Hit
ATTGGACGTATCGCATTTCGGCAGCGTT329570.13710914253014772No Hit
CCACTACCATTAGCCAACTGACAATTTT329480.13707170033933025No Hit
CAGATCACTCAGATCACGTAGGACTTTA327880.13640606139146413No Hit
ATCACGAAGGAGCGAGCTGGTGAGATTT327760.13635613847037417No Hit
CAGTAACCATTTGCCCGTAGATTGGGTT327350.13618556848998348No Hit
TGACAACCATTATCTCCCACCCAGGGTT326500.13583194779892963No Hit
GCTCTGTGTAGCGATGTTAGTTGGGATT325010.1352120715287293No Hit
TTGGGCATGTTTACATGCATGTCAGGCA322900.13433426016623085No Hit
CATTCGCTACCTTAGGACCGTTATAGTT322710.13425521554117176No Hit
CGTCCATGTTAGAACATCAAACATTAAA322570.13419697213323345No Hit
CAGCAGCCAGGAATCGATGAATGGCCTT318610.1325495157372648No Hit
GCAAACTCACATGACTGCTGGTAAAATT318200.13237894575687412No Hit
GAACATCTCAACGCTACAGTTCGCGCTT317220.13197124190130613No Hit
GACTGCGAGACTAGATCGGACCATGGTT313810.13055259889366647No Hit
CTAATGGCAGTGGAGTACAGCACGACTT312580.13004088895249438No Hit
GGCGATTCCGATATGCCTGTTGCGCTTT311170.12945429462968736No Hit
GACTGCGCCACTGGATTTCAAGACTTGC310580.12920884026766175No Hit
GACTGGATATATTTTATGGGCATGTAGT309360.12870129056991383No Hit
CTTTGCGCTGTTATCCCTAGGGTAACTT303270.12616770232459842No Hit
AGCGGCTGCACCATCGGGATGTCCTGAT301770.12554366581097395No Hit
AACCATGGTTGTACACCCAGGATCCGTT301420.1253980572911282No Hit
CGCAGTTCTCCGCTTCTTGGCCTCACTA296000.12314320535523175No Hit
GTAACTGCATCTACGACTTGCTATCCTT292860.12183688892004449No Hit
TGCAACCATTATCTCCACGGTTTCGTTT291560.12129605727490328No Hit
CAGCAGCCAGGAATCGTTACCCACGCTT287880.1197650876948112No Hit
GGCAGTAGGTGATTATCCAAACACTTTT287180.11947387065511976No Hit
CACCTTGAGTGAAGAGTCGTCCGACATT287020.11940730676033318No Hit
CATTCGCTCTCAAACGTGGAAGAAGGTT286850.11933658262212239No Hit
GCGCAGTAGGTGATTAAGATTTCTGTTT286030.118995442661341No Hit
AACATCTCAACGCTACTACTGCTTCTTT285930.11895384022709936No Hit
GACTGCGAGACTAGATCGTTGTACTAAT283640.11800114448296598No Hit
ACGTCAATCTGCAAGTTTGATGGTGCTT281400.11706924995595343No Hit
CAGCACGTTTCTTCTCATTCATTTCCCA280700.11677803291626199No Hit
GAACATCTCAACGCTACCCGACGGCATT279380.11622888078427245No Hit
CAAACTCACATGACTCTATACGTTATTT278960.11605415056045759No Hit
GACCATCTCAATGTCAGTGATTACGTCG277650.11550915867189221No Hit
CACCTTGTCATTAGTGATAGTAATCTTG277360.11538851161259148No Hit
CGACCTTTCACAGGCCTCCTAGGTCGTT275240.11450654000666888No Hit
TAGCCGGTCGTTTAGGTCATGTAGGGTT274470.1141862012630083No Hit
CGTCCATCAAGAGTCGTTCAACCAATTT274180.11406555420370758No Hit
GATCAGTCACGACGAACTGGACAGCTTT274140.11404891323001091No Hit
GCGTGGGTATAATACTAAGTTGAGATGA273690.11386170227592357No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG273380.1137327347297745No Hit
TCGGGACAGGTAGCCACCAGCTGCGCTT272550.11338743452556897No Hit
GCTGGGTGTGGGTATGCTAGTCGTCCTT270730.11263027022237126No Hit
ACACGTAGGACTTTAATCGTTGAACAAA268880.11186062518890105No Hit
CCTTTCTAGATGCGACATAGGTTTCGTT268690.11178158056384195No Hit
CATTCGCTCCTATGTTACCACAATACAT267120.11112842234624833No Hit
ACTTACTCACAGGTTTCGTCTTGCGTTT266320.11079560287231525No Hit
CCTTTCTTCTAAAATAACCCCTGTGTCC261610.10883612821953438No Hit
CACCTTTGCAGTAACACCAAGTACAGGA261270.10869467994311284No Hit
GGCCTTGGCCTCTCTGCCCCTCCAGCTG256780.10682673064566353No Hit
CGACCTTAGTCAATAGCTCTCGATGATT256540.10672688480348362No Hit
AACTCAGCACGCATCGCTCAAAGACGTT253310.1053831261774789No Hit
CCTTTCTTTCTGAAGGTTTTACGATGCA249550.10381887464999352No Hit
GCGCAGTAGGTGATTATACTCTTCGGTT248000.10317403691924822No Hit
GGCCATTTGGGGCATTCATCCCATCTAC245950.10232118701729476No Hit
CGGAGTCCAGAACAGGAAGTGGGTCCTT245890.10229622555674978No Hit
GACTGCGAGACTAGATAACGTATCTCTT245730.10222966166196316No Hit
GACAATAGCGTTTACTTATCATGCCTTT245220.10201748924733084No Hit
GCACTCTTTGAGCTGCTGCACACAGTAC244450.10169715050367027No Hit
ACCGTAAGTCACACGCGAACGGGGTGTT244250.101613945635187No Hit
TTGCGCTTTGTGAAGTAGGCCTTATTTC243520.10131024786522308No Hit
CACCTTGAGAGGGATAACACCACAATTT242300.10080269816747517No Hit
CACTCCAGTGTTGGGAGCCGACGTTGTT242090.10071533305556775No Hit
GACTGCGAGACTAGATGTACGATACTTT241580.10050316064093541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)