FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24037055
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3183091.324242924101975No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2750321.1442000694344627No Hit
GGTTCTGTGAGTCCTGCTTTCAAGAGACTAAAAATTGCCCAGCATACGAG255690.10637326411242976No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC249480.10378975294602437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG385400.066.635351
ATACGGC17300.055.1711245
TACGGCG21650.043.5109866
TGATACG22550.042.6925933
GGCTCAA774350.037.7764556
CGGCGAC24150.037.4604538
GCTCAAA790150.037.0263947
CTCAAAC808600.036.1765758
TCAAACA822200.035.5832259
GAACACG741850.034.88807732-33
CAGCGAC761800.034.70421616-17
ACACGTT750100.034.27573834-35
ACGTTTT753650.034.2629936-37
TCGGGTG783650.033.6282424-25
GTCTCTC112000.033.506192
GACCGTG768150.033.45413256-57
CCTCGGG787650.033.41794622-23
GGGAACA795700.032.9907930-31
TGGCTCA924100.032.0319445
CCGTGAG803600.032.00174358-59
Produced by FastQC (version 0.11.5)