FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24704142
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3368921.3637065395754282No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2920811.1823159047579956No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC265670.10754067071019913No Hit
GGTTCTGTGAGTCCTGCTTTCAAGAGACTAAAAATTGCCCAGCATACGAG256000.1036263473550306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG400000.068.489331
ATACGGC17400.052.231145
TGATACG22700.040.5831643
TACGGCG21650.040.061276
GGCTCAA811750.037.2275736
GCTCAAA833350.036.262517
CGGCGAC23350.036.078118
CTCAAAC852550.035.465328
TCAAACA865550.034.9709439
GAACACG784550.034.7502432-33
CAGCGAC807200.034.40524716-17
ACGTTTT794250.034.22245836-37
ACACGTT794350.034.04812234-35
GTCTCTC120150.033.8911442
TCGGGTG829700.033.4067124-25
GACCGTG814300.033.15756-57
CCTCGGG836500.033.12770522-23
GGGAACA837150.032.9559530-31
AGTCTCT125400.032.256171
CAAACAC905350.031.9440110-11
Produced by FastQC (version 0.11.5)