FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26002028
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAA1745300.6712168758529142No Hit
ATTGGACGTAGCTAAAGGGGTCTCATTT1219400.46896342085317344No Hit
GACTAACGTACATCCACCCAGATGTGTT1099540.4228670163727229No Hit
TGGCAGGTAAGTAGTAAGGCTGCTATTT994620.3825163175733831No Hit
TGCCCTACAGTGGAGTATAAAGCGCGTT955100.36731750308091354No Hit
CGTAGGCGTTCCACAAGCTTGATGTATT952520.36632527278256916No Hit
CCTTTCTGTATTCTCTGGTGGTCCTCTT925750.35602992197377836No Hit
GGATTACTCCGGTCTGAACTCAGATCAC816950.31418703187305236No Hit
CTAATGGTCCTGTACCCTTTTGTATATT774750.29795752854354285No Hit
CGGACTGTCATGTCTTATATCAATCGTT764930.2941809000436427No Hit
CTAATGGTCCTGTACCGAATACATTATT748370.2878121660356646No Hit
ATTGGACTCACGAAGGTATAATCGATTT703830.2706827329006799No Hit
TGACTTTTACTTCCTCTAGATAGTCAAG696330.26779834249851586No Hit
CTAATGGTCCTGTACCAAGGGAATTGTT662360.2547339769036477No Hit
TCATTACAGACTAAGTGGTACACTCCTT650740.2502650947072282No Hit
AGGTCATCACACCGACAAGCGGGTATTT624230.24006973609904583No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA598820.2302974214165141No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG556440.21399869271735267No Hit
GACGCGTAGGCGCTCTCAACACTGACTT547400.21052204081927767No Hit
CTCGTCATCCTCTAGCCGCCTTAGGATT541930.2084183587526327No Hit
CAAGTTGAGGAGTCTGAACGGGGTATTT525010.20191117400535064No Hit
AGTGGGTATAATACTAAGTTGAGATGAT492080.18924677721291586No Hit
TAGAGCTGTGGACGATTATACTCGTATT486800.18721616636979238No Hit
TGAGCCGGTATATGAGGTTCCCCGTTTT475480.1828626597894595No Hit
AGTGAGGGTAATAATGACTTGTTGGTTG443240.17046362691402378No Hit
TAGCCGGTCACCGTAACAGGCCAGCCTT393060.15116513219661173No Hit
TTCTTAGCATCACCCTCATGTGTCGCTT386150.14850764717275128No Hit
CGGAACATGTCGCTTTCGCTTTACATCT382170.14697699733266958No Hit
CAGCTGGAGTAGGTGCTATCTCTTCTTT362550.1394314320406085No Hit
CAGATCACGTAGGACTTTAATCGTTGAA351020.13499716252901506No Hit
CGATTGATCGAATCCACCCGGCTCCTTT346830.13338574975767276No Hit
AGTGGGACAGGACCCTAGCATCACGGTT343190.13198585894915582No Hit
GTTTCTACAGGTGCCTTGCTAGTATTTT335390.12898609293090524No Hit
AGGGGTTTTAGTTAAATGTCCTTTGAAG305280.1174062269296841No Hit
CATGCCTTCGCAAGCCCTCTAGTAGGTT305110.11734084741390173No Hit
AGAGTGGGTATAATACTAAGTTGAGATG297890.11456414092008516No Hit
GAAATGACATATGAGATCGATTAATGTT297330.11434877310339024No Hit
AGGCCACAGTCTCGGCGCGATACAATTT296350.11397187942417414No Hit
TTGTAGGGGTTTTAGTTAAATGTCCTTT284900.10956837674353707No Hit
GCTGGGTATAATACTAAGTTGAGATGAT280000.10768390834745659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG116450.021.1520652
CTAATGG302300.021.0302871
AGACTAA133350.020.9275328
CGGACTG133800.020.9084051
GCTAAAG136700.020.81565511
TAAAGCG101200.020.81543718
TTGGACG140900.020.7842942
GACTAAG134400.020.7217589
TGCCCTA195400.020.6619911
GACTAAC130700.020.629961
TCCTGTA307100.020.6065438
TATAAAG102400.020.5929916
AAGCGCG102100.020.5873520
AACGTAC120000.020.5063635
TGAGCCG144750.020.5047281
ACGTAGC143000.020.500536
TAATGGT289700.020.4833322
CAGACTA141000.020.3856357
TAATCGA79950.020.37505319
AATGGTC291650.020.2860263
Produced by FastQC (version 0.11.5)