FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26002028
Sequences flagged as poor quality0
Sequence length89
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1706690.6563680340625739No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1486820.5718092450327337No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC604730.23257032105341935No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC559530.2151870615630442No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG271490.10441108670446783No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG267240.1027765988099082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG337350.061.529541
AGTCTCT246900.056.181451
GTCTCTC261350.053.332362
TCTCTCA274850.050.773213
CAGCGTA35650.044.234289
CTCTCAG366650.038.2306754
TAAGTTC46750.035.4261443
GCTCAAA590400.035.23688
CTCAAAC607700.034.307999
GATGGCT612600.034.108994
GTAAGTT48650.033.8719562
CTGATGG619850.033.629682
TACACGG231800.033.47086714-15
AGCTGGT422900.033.3932849
ACACGTT422450.033.320834-35
TCGGGTG569600.033.10576624-25
CAGCGAC590450.032.77750416-17
ACGTTTT433150.032.7566836-37
CAGCTGG431800.032.6642388
CACAGCG595950.032.56960314-15
Produced by FastQC (version 0.11.5)