FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23790056
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTGGTCAGACGCAATTCCTACTGCTT1030190.4330338692771467No Hit
CAGTAACAGCATCATCTATTCTGTTTTT965590.4058796666977161No Hit
TCATTACAGATATGCATTTCATTCCTTT928630.39034376379778174No Hit
ATTGGACGTATAGGTATATTTGCTCTTT909800.3824286920552015No Hit
CTCATTATCAGTTTGGACACCTACGGTT903220.37966283055407685No Hit
TGCGTGGAGCACACAGCTCCTTATAATT829500.3486750934928442No Hit
GATCAGTGTATAGGTAGTCAGCCGAGTT772980.3249172679543083No Hit
TCATTACAGATATGCACCCTCGCATATT724730.3046356847583713No Hit
TGCACCTAGTCTCCTCAATTCTTATGCT719650.3025003387970167No Hit
CGATTGAGTTACAGAACTAAGACCATTT708060.29762855539306No Hit
GCTGGGTAGGCCATAGTCACCTAAGTTT670620.28189088752039926No Hit
ATCATGGTCCTATTCAATTGTACACCTT641410.2696126482426103No Hit
GATCGATGTTAAAGTGGGCGTTCAGTTT628260.2640851286772927No Hit
CGTTAGAAGCTAACAAGTCCGATTTATT606420.2549048224182406No Hit
CCCAATCGTGAGGGTTTTGGGCGCGTTT603820.25381192881597253No Hit
AGTTTTGTCTCTTTATGCGTCAGTGTTT599220.2518783478273443No Hit
TGCGTGGCAAAGGAAGTTAGACGGATTT593620.2495244231455361No Hit
TGGCTGGGTGTAATGATGTAGTTCCTTT571320.24015075878762118No Hit
TCATTACAGATATGCAAGCCATTGTCTT506020.21270231562296446No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA503120.21148331891274239No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG473360.19897389060370435No Hit
TGCGTGGAGCACACAGCTGTTGTATATT463150.19468218149633612No Hit
CTAAGACCAAGGTTCTTTTTACGCTCTT461540.19400542815031624No Hit
TAGCCAGTAAGTGTAGGTTTTTTGGTTT422760.17770449972879426No Hit
TGATTTCAGAGCCCAACGCAGGTCCTTT411120.17281169914017858No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA382510.16078566607829758No Hit
CCTTCCCCTAAATGATAAGGTTCAATGA364540.15323208991185225No Hit
TACTTGTCATAGACTCTCAATTGCTATT343390.14434182080109437No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGT342900.14413585239143614No Hit
CAGTAACAGCATCATCGGGCCAGCACTT335900.14119344653917587No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGA318120.1337197356744347No Hit
CAGATCACGTAGGACTTTAATCGTTGAA312930.13153815190683033No Hit
CTACCCAGTGGTACAGGGGACGTATGTT291430.12250076250345944No Hit
GGATTACTCCGGTCTGAACTCAGATCAC266280.1119291186199814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATG283850.021.0733558
CAGATAT270250.021.0226767
TGCGTGG308400.020.9596791
GCGTGGA171850.020.9399012
GTGGAGC165800.020.7935624
GTATAGG264000.020.7912228
ACAGATA269200.020.758846
AGTAACA165500.020.7395942
GATATGC280800.020.7342439
ATATGCA281550.020.71790510
TGGAGCA165800.020.6341975
TAACAGC164250.020.6013374
TATAGGT249700.020.5854639
ATTACAG287350.020.5769823
CATTACA277150.020.5577092
GTAACAG166850.020.5506253
GAACTAA76150.020.51136214
TCAGACG116500.020.488837
ATAGGTA251650.020.45637510
CTTATAA91050.020.40449120
Produced by FastQC (version 0.11.5)