FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23790056
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC966990.4064681478681681No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC624800.2626307394988898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG507640.21338327240591615No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAGCCAGT326010.13703624741362525No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC265870.11175677770577756No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA259990.10928515678987893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT227900.056.9972721
TCTCTCA244150.053.253783
TCTGATG210700.051.283121
GTCTCTC263700.049.5035932
ATACGGC26300.047.9697385
TACGGCG23200.047.939816
CTCTCAG293950.044.5707324
TGATACG31050.044.507113
CTCAGCT294900.044.328736
TCTCAGC322600.040.599645
AGCTGGT339100.038.7337079
CAGCTGG357850.036.9593358
GCTCAAA298150.036.384298
TCAGCTG364300.035.9867
CTCAAAC302150.035.943829
GATGGCT307600.035.280444
TACACGG197500.034.5339114-15
CTGATGG320550.033.990672
TCGGGTG265400.033.2193224-25
GGCTCAA328600.033.1516277
Produced by FastQC (version 0.11.5)