FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004724475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23514852
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTGGTCAGACGCAATTCCTACTGCTT1023480.43524832731245766No Hit
CAGTAACAGCATCATCTATTCTGTTTTT950270.4041148122046441No Hit
TCATTACAGATATGCATTTCATTCCTTT925560.3936065598031406No Hit
ATTGGACGTATAGGTATATTTGCTCTTT891280.3790285390696909No Hit
CTCATTATCAGTTTGGACACCTACGGTT890470.37868407591933817No Hit
TGCGTGGAGCACACAGCTCCTTATAATT823980.3504083291700071No Hit
GATCAGTGTATAGGTAGTCAGCCGAGTT766300.3258791507596986No Hit
TGCACCTAGTCTCCTCAATTCTTATGCT718260.30544950910173707No Hit
TCATTACAGATATGCACCCTCGCATATT711960.3027703512656597No Hit
CGATTGAGTTACAGAACTAAGACCATTT704190.2994660566011642No Hit
GCTGGGTAGGCCATAGTCACCTAAGTTT666180.28330180432349733No Hit
ATCATGGTCCTATTCAATTGTACACCTT637350.2710414677498289No Hit
GATCGATGTTAAAGTGGGCGTTCAGTTT623730.2652493836661188No Hit
CGTTAGAAGCTAACAAGTCCGATTTATT600110.2552046680965715No Hit
AGTTTTGTCTCTTTATGCGTCAGTGTTT593110.2522278260564855No Hit
CCCAATCGTGAGGGTTTTGGGCGCGTTT592640.25202795237665115No Hit
TGCGTGGCAAAGGAAGTTAGACGGATTT583520.2481495524615677No Hit
TGGCTGGGTGTAATGATGTAGTTCCTTT558170.23736913164497062No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA499770.21253376376768182No Hit
TCATTACAGATATGCAAGCCATTGTCTT493810.20999919540212286No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG460370.19577839571348357No Hit
TGCGTGGAGCACACAGCTGTTGTATATT459710.1954977220354183No Hit
CTAAGACCAAGGTTCTTTTTACGCTCTT448930.1909133852936859No Hit
TAGCCAGTAAGTGTAGGTTTTTTGGTTT412920.1755996593131864No Hit
TGATTTCAGAGCCCAACGCAGGTCCTTT404350.17195515412982398No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA380430.1617828596157016No Hit
CCTTCCCCTAAATGATAAGGTTCAATGA357530.1520443335131346No Hit
TACTTGTCATAGACTCTCAATTGCTATT339920.14455544946657542No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGT337510.14353056527848868No Hit
CAGTAACAGCATCATCGGGCCAGCACTT330400.14050694429205848No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGA312510.13289898656389587No Hit
CAGATCACGTAGGACTTTAATCGTTGAA307210.1306450918764022No Hit
CTACCCAGTGGTACAGGGGACGTATGTT294060.12505288147252638No Hit
GGATTACTCCGGTCTGAACTCAGATCAC265540.11292437647491892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)