FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004724477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17999698
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAA1231560.6842114795481569No Hit
TCAGGATTCAAGATCCCCCCTTCTCATT859990.47778023831288724No Hit
CCACCTCAATCCGATCTTAGGGCGCTTT813870.4521575861995018No Hit
CGTCTACAGCTGCCCAGCCTCATTCTTT802460.44581859095635934No Hit
ATAAGAGTCTGCAGTACCGCCCGCAGTT786270.4368239956025929No Hit
GTACGTAGCTAGTCTAGAATCGATTTTT725630.4031345414795292No Hit
TCAGGATTCAAGATCCATGAAACCTATT719690.3998344861119336No Hit
TCTCTAAAGGGTCTCCACAGGTCTAATT678220.37679521067520133No Hit
GTACTCCTCCCGGATGGTCGCAATACTT660400.3668950445724145No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG624600.3470058219865689No Hit
GTATCTTTCACTGGGCAAGACCCTGGTT619160.3439835490573231No Hit
ACCAGTAAGCTGATAACACTCGTTAGTT599730.33318892350304985No Hit
ACCAGTAAGCTGATAATCCTGCCATATT583320.3240721038764095No Hit
CAGTATAGTATTCGTGTTTATCTCTATT543060.3017050619404837No Hit
CATGACAGTAAGGATTGTACACCTACTT542810.30156617072130876No Hit
GTCATTTGTATTCGTGCCAAGAGTTGTT530860.2949271704447486No Hit
GATCTAGTCACTTACTTACATATCTTTT520330.2890770722931018No Hit
CCCAGTTCACCCAGTGCCAGGCTTCTTT506640.2814713891310843No Hit
AAAGTAGCAGCGAACAATTTCGTCAGTT493440.2741379327586496No Hit
ATTGGTGAGTAGATGTAGGGTGCGCCTT485860.2699267509932667No Hit
ACGCAGCGTCAACATCATAGCTTGTCGT478810.2660100186125345No Hit
CTCCTAGAGAGTAAGGAAGGCGAGAATT432120.24007069451943028No Hit
GATCTAGTCACTTACTGCCGCATCGCTT423910.2355095068817266No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGA415540.23085942886375094No Hit
GGATTACTCCGGTCTGAACTCAGATCAC408640.22702603121452372No Hit
GATCTAGTCACTTACTTCTCAGACAGTT368440.20469232317119984No Hit
TTCCCAGAGCGTAATAGTTGGTTAATTT347390.19299768251667332No Hit
GGATGTTAGATGTTAGCTCCCTCCGGTT337330.18740869985707537No Hit
TACCTATAGTTTGCGTTATCTGTGGGTT333510.18528644202808292No Hit
TGTATTCAGATCACGGCGTCCGGGCCTT306130.17007507570404792No Hit
TATTACCCAGTAAGATAGTAATCCCTTT306020.17001396356761098No Hit
TCTCTAAAGGGTCTCCGCACCGTACGTT300400.16689168896055923No Hit
CTACATTGTTACTGACGGCATGCTTTTT277520.15418036458167245No Hit
TCTCTAAAGGGTCTCCTACTACTCGTTT274330.1524081126250007No Hit
GATCTAGTCACTTACTTGTTCTACCGTT270800.15044696861025111No Hit
CAGATCACGTAGGACTTTAATCGTTGAA267810.14878582962891934No Hit
GCCTCCTACACGGCCATCCACCAATTAT266720.1481802639133168No Hit
TACTCATTCGTGGGAACGGTGTCCGATT256770.14265239339015579No Hit
TCAGCTCGTTGGAGGTTTTACAGTCCTT255010.14167459920716446No Hit
TGCTACCTTTGCACGGTTAGGGTACCGC250890.13938567191516213No Hit
GTACTCCTCACTCTTAGGATCAGGGCTT249760.13875788360449157No Hit
GCCTCTATATAAATGCGTAGGGGTTTTA225040.12502431985247753No Hit
ATTGGTGAGTAGATGTGGTGCGCCAGTT218650.12147426029036709No Hit
GTACTCCTCACTCTTAGCACGCTCATTT218620.1214575933440661No Hit
ATTTCTGTCAAGAAGTTTACTCTATCTT216990.12055202259504576No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA208460.11581305419679819No Hit
GCATGTAGTCTCTTTAAGGCGAATCCTT189730.10540732405621472No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT183940.10219060342012405No Hit
CAACTAGTCATAGCACCCCTATTTCGTT182390.10132947786123966No Hit
TTGTAGGACTTTAATCGTTGAACAAACG180680.10037946192208336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)