FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004724479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188500
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCCGTTGGTCAAGTTATTGGATCAA1254110.6895071061384941No Hit
TCAGGATTCAAGATCCCCCCTTCTCATT863780.47490447260631713No Hit
CCACCTCAATCCGATCTTAGGGCGCTTT824690.45341287076999204No Hit
CGTCTACAGCTGCCCAGCCTCATTCTTT805320.4427632844929488No Hit
ATAAGAGTCTGCAGTACCGCCCGCAGTT803080.4415317370866207No Hit
TCAGGATTCAAGATCCATGAAACCTATT734250.403689144239492No Hit
GTACGTAGCTAGTCTAGAATCGATTTTT732660.4028149655001787No Hit
TCTCTAAAGGGTCTCCACAGGTCTAATT681450.3746598125188993No Hit
GTACTCCTCCCGGATGGTCGCAATACTT670950.368886934051736No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG630620.3467135827583363No Hit
GTATCTTTCACTGGGCAAGACCCTGGTT629360.34602083734227673No Hit
ACCAGTAAGCTGATAACACTCGTTAGTT608010.3342826511257113No Hit
ACCAGTAAGCTGATAATCCTGCCATATT589720.32422684663386203No Hit
CATGACAGTAAGGATTGTACACCTACTT548560.30159716304258183No Hit
CAGTATAGTATTCGTGTTTATCTCTATT548030.3013057701294774No Hit
GTCATTTGTATTCGTGCCAAGAGTTGTT536070.29473018665640377No Hit
GATCTAGTCACTTACTTACATATCTTTT523430.28778074057783765No Hit
CCCAGTTCACCCAGTGCCAGGCTTCTTT515110.2832064216400473No Hit
ATTGGTGAGTAGATGTAGGGTGCGCCTT490040.2694229870522583No Hit
AAAGTAGCAGCGAACAATTTCGTCAGTT488560.2686092860873629No Hit
ACGCAGCGTCAACATCATAGCTTGTCGT486540.2674986942298705No Hit
CTCCTAGAGAGTAAGGAAGGCGAGAATT437290.24042114522912827No Hit
GATCTAGTCACTTACTGCCGCATCGCTT426640.23456579706957692No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGA422770.2324380790059653No Hit
GGATTACTCCGGTCTGAACTCAGATCAC410020.22542815515298126No Hit
GATCTAGTCACTTACTTCTCAGACAGTT375010.206179728949611No Hit
TTCCCAGAGCGTAATAGTTGGTTAATTT344710.18952085108722544No Hit
GGATGTTAGATGTTAGCTCCCTCCGGTT339190.1864859664073453No Hit
TACCTATAGTTTGCGTTATCTGTGGGTT337940.18579871897077824No Hit
TGTATTCAGATCACGGCGTCCGGGCCTT307190.16889243203122853No Hit
TATTACCCAGTAAGATAGTAATCCCTTT307090.16883745223630317No Hit
TCTCTAAAGGGTCTCCGCACCGTACGTT305370.1678917997635869No Hit
CTACATTGTTACTGACGGCATGCTTTTT287740.15819886191824503No Hit
CAGATCACGTAGGACTTTAATCGTTGAA274160.15073260576738048No Hit
TCTCTAAAGGGTCTCCTACTACTCGTTT274030.1506611320339775No Hit
GATCTAGTCACTTACTTGTTCTACCGTT272460.14979794925364928No Hit
GCCTCCTACACGGCCATCCACCAATTAT268800.14778568875938092No Hit
GTACTCCTCACTCTTAGGATCAGGGCTT258230.14197432443576985No Hit
TACTCATTCGTGGGAACGGTGTCCGATT256680.1411221376144267No Hit
TCAGCTCGTTGGAGGTTTTACAGTCCTT256320.1409242103526954No Hit
TGCTACCTTTGCACGGTTAGGGTACCGC254900.1401434972647552No Hit
GCCTCTATATAAATGCGTAGGGGTTTTA230470.12671193336448855No Hit
GTACTCCTCACTCTTAGCACGCTCATTT220330.12113698215905655No Hit
ATTGGTGAGTAGATGTGGTGCGCCAGTT220130.12102702256920582No Hit
ATTTCTGTCAAGAAGTTTACTCTATCTT215430.11844297220771366No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGA210760.11587541578469912No Hit
GCATGTAGTCTCTTTAAGGCGAATCCTT192700.10594606482117822No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTT187390.10302663771064133No Hit
CAACTAGTCATAGCACCCCTATTTCGTT183360.10081095197514912No Hit
AGGGAGTCACATTTCTTCCCACGCGATT182110.10012370453858206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)