FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188500
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1325040.7285042746790554No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC864150.47510789784754104No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC855140.4701542183247656No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC552310.303658905352283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399520.21965527668581794No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT197400.10853011518267036No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG195080.1072545839404019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG286950.068.077051
AGTCTCT189050.061.155031
GTCTCTC204600.056.478232
TCTCTCA221600.052.1552053
CTCAGCT247200.047.0064856
CTCTCAG256050.045.3006064
ATACGGC12000.044.9583445
TACGGCG9650.044.2953456
CAGCTGG262700.044.2008938
TCTCAGC271100.043.0155035
GATGGCT455550.042.9529154
AGCTGGT272500.042.8397259
GCTCAAA463000.042.333138
GGCTCAA465900.042.0339977
CTCAAAC467800.041.9165049
CTGATGG495100.039.5616572
TGATACG14700.038.676353
TGATGGC528700.037.0724143
CACGGCA169550.035.61285816-17
GGTACAC175900.035.3778312-13
Produced by FastQC (version 0.11.5)