FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12361144
Sequences flagged as poor quality0
Sequence length89
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1234440.9986454328175451No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC907450.7341149006920394No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG505950.4093067761365777No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC503440.40727621974147377No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA440220.35613208615642694No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG377560.30544098507387346No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC373890.30247200420931913No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA298820.2417413792768695No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC230710.1866413011611223No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC203170.16436180987779125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203130.16432945041332744No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC151770.12277989804179937No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT138050.11168060173071359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT360850.073.67681
GTCTCTC395800.067.0624852
TCTCAGC458300.058.1156435
TCTCTCA458850.058.081933
CTCTCAG460200.057.9298024
TCTGATG263500.057.8961941
AGCTGGT469450.056.6739169
CTCAGCT476900.055.8318526
CAGCTGG484850.054.9422848
TCAGCTG525050.050.7038577
ATACGGC6750.043.029855
CTGATGG361150.042.4067962
GGCTCAA365250.041.9646847
GCTCAAA366850.041.7931378
CTCAAAC377800.040.5488749
TGATGGC388900.039.4230583
GATGGCT389850.039.3803634
TGGCTCA393200.039.0555656
ATGGCTC407300.037.7441255
GGTACAC408150.036.9749712-13
Produced by FastQC (version 0.11.5)