FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004724493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9035323
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCTAGAGTACCGTCATCCTCGTTT1913122.1173786482232013No Hit
AACCGCGGGAACCCCGCATCTTCACGGG1689091.8694295710291706No Hit
TGGCTGGAGCTGATAATAGCCCTAACTT1558671.725084980359861No Hit
CACCTCAGCAATATGATCTAGTGGATTT1327631.4693774644249022No Hit
GTCCTCAAGGTCATCTCTCACACGCATT1326181.4677726518465362No Hit
GCAAACTTCATCGCTCACTTTGTCCATT1260361.394925228461672No Hit
GACGGCTCTATGAAATCTTATCTCGAGG1057121.1699858433395243No Hit
CAGCTAAAGCCGGTAATATAATGAACTT1041191.1523550403234062No Hit
TGGCTGGAGCTGATAACTAGTGACATTT1014801.1231474513971444No Hit
AGTGGGAAGAGGTAGACGTGGTCAAATT1002031.1090140330345688No Hit
ATAACGCGTTCCACTCCCCGAAGGCGTT990101.0958102992001502No Hit
GATCGTAGTGAGGGAGTAGTACGGGATT973531.0774711651149604No Hit
CAAGAAATCGACAGCCAAACCAGTAGTT912091.0094713824840573No Hit
TGGCTGGAGCTGATAAACATAGGCGGTT817280.9045387752048266No Hit
TCATTACCACGTAAGGGTGTGTTACGTT800580.8860557613712315No Hit
CCTCAGTAGCGTTGCCAAGATGTTCTTT788370.872542132694094No Hit
TGGCTGGAGCTGATAAAACTTTCCTTTT738910.8178014222623807No Hit
TCTGAGATCTGCGTAACCTTCTGCCGTT733050.811315765911191No Hit
AGTTGGTAGCCGGTAAAATGAAGTATTT729560.8074531480501582No Hit
CAGCTGGAGCCCGAAACGTCAGCCTTTT721010.7979902876742757No Hit
AGCTCCTAGAGTACCGCGACAAAACCTT702450.7774486866711904No Hit
TCTGAGACAACTGCTAAAGTCTTATCTT662770.7335321603887321No Hit
GTTCATTTCGTCCAGGTACATTGTAATT661430.7320490922128627No Hit
GCTGGGTAGTAACCCTGTCTATCAGGTT613420.6789131943595155No Hit
CGTAGCGAGTTCGCATTACATTGGACTT605570.6702250710904303No Hit
TGGCTGGAGCTGATAACTCATTAGCTTT591860.6550512914701555No Hit
CAGCTGGTCTGCCAGGGAAGTTCGGGTT536620.5939134660708865No Hit
GGCGACTAGGACATTACGGGAGGTGGTT510610.565126448716886No Hit
CAGCAGCGGCCTTTGTTTTCAGACCCTT504330.5581759501016179No Hit
TACTCGCTCTTAACCTTGTTATGCGTTT490640.5430243058272516No Hit
ATGTGTGGTACGAAATGACCACGCCGTT480890.5322333246968591No Hit
CATATTCAGTTTCCTTCCTGTCGTCCTT462220.5115699792912771No Hit
TCTGAGACAACTGCTAATTTTTACTGTT452240.5005244416829371No Hit
ATTGGTGTCCAGTAGTCAACGGTCATTT437570.48428816545905445No Hit
GGACAGATCGGTTAACGAGTGGAATCTT413230.45734944948841344No Hit
TAGGCATTCTGTTGAGAGGCGTGCCGTT390450.4321372904986352No Hit
TGGCTGGAGCTGATAAAAACATTGGGTT367810.40708007893021647No Hit
TACCTTAAGATGTGTAGGCATGCACTTT341860.37835946761394146No Hit
CTACATTAGAATGTGTCCGGGGCCAGTT338120.37422015792905244No Hit
CGATGGCGTTTACTCTGGGTGTATCGTT335360.37116548019367984No Hit
CGTCCATACATAGCTACCCTGCTGCGCT327480.362444153905732No Hit
TAGGCATTCTGTTGAGGTGGGAGGCGTT307680.34053016145631987No Hit
TAGACCATCTCTTATGTTCCATCCGGTT289110.3199774927802802No Hit
AGTGTCATCCTTAATCACGGTCCGCTTT286190.31674573227763964No Hit
CTACATTAGAATGTGTAAAAAGGCCGTT258290.2858669247352862No Hit
ATCCTACTTCTAACTCTGAGTCACCGTT247720.27416839442264546No Hit
ATCTACTGTACAAGTAATTTCGCTACTT240760.26646529404648844No Hit
AGAATAGAGACTAGATTCGCCGCCTATT235830.26100893128004393No Hit
TCTGAGAAGGATGTATGAACTCCTGATT216490.2396040517865272No Hit
TGGCTGGAGCTGATAATAGCCACGGCTT206570.2286249202159126No Hit
TCTGAGAAGGATGTATCCGTCTCGGCTT188980.20915688348938938No Hit
GAACATCAGGAATTACGTCGACTCGTTT188520.20864777053349395No Hit
ATTACTCGTGTCAATCCGGGGGCTTCTT187700.20774022135124554No Hit
ATCTACTTCTAACTCTTCTCTCCCTGTT185020.20477408499950692No Hit
GCGGGTTTCGAATCCACCTGGCAAAATT182230.20168620424527156No Hit
GAACATCAGGAATTACATATTAACGGTT170270.1884492673919903No Hit
TACTTGTAGATGTGGCTTACTAGGTTTT164720.18230670890238235No Hit
TGACGGCTCTATGAAATCTTATCTCGAG163560.18102285883968952No Hit
TACACGAGTAATAGCAGAGTTTCTTTTT161730.17899747468906202No Hit
CCACTACCCATTACCAGATCGCTTCACA161150.1783555496577156No Hit
AAGGTTCGTGTTGGGATGTAACGTAGTT152690.16899229833842133No Hit
GACTGCGGGAACCCCGCATCTTCACGGG151940.16816222286685267No Hit
TCTCAAGTCTCTCTGTAGAGCGGGCTTT151760.1679630047536762No Hit
TCATTACTCCCAAGTAGTGTTTAGACTT133420.14766489255558435No Hit
CTCGAGGGAGGCTTCCCGCTTAGATGCT130610.1445548764554405No Hit
ACATCAGTCACTTATCAAATCAAGCCTT130500.14443313205294378No Hit
AGTGGGTATAATACTAAGTTGAGATGAT129420.1432378233738849No Hit
TATTACCGTAAGTAGTCAAAAGTTGCTT128790.14254055997776727No Hit
TTGGAACCAGGGATTGGGTCTATCGCTT128020.14168834916029013No Hit
TTCAACGATCTAGCACATGCGTTGTGGT124760.13808028777720507No Hit
TATTACCGTAAGTAGTGAAGTTCTTGTT119570.13233616551395008No Hit
GCTTGAATCTTTAGTCTAACCTATGATT114820.127079020860682No Hit
ACTGATGCAATGAAACGCTCCATCCTTT114740.12699047947704803No Hit
AAGCCGCGGGAACCCCGCATCTTCACGG108780.12039414639631588No Hit
AACCATGGGAACCCCGCATCTTCACGGG107100.11853477734000213No Hit
TAGGCATTCTGTTGAGGTTCTTCAATTT106090.11741694237162302No Hit
TCTGAGAAGGATGTATCGTATTTATCTT104570.11573465608257724No Hit
TTCTCAAGTCATACTGCATGTTATGGTT104480.11563504702598901No Hit
ATCGAGTCATGGTCTCTGACGGCTCTAT95700.10591763017215876No Hit
TAGGCATTCTGTTGAGCGCTACCCAGTT95330.10550812627285157No Hit
GGGCATCATTTCAGAGCTTGCACCCCTC93700.10370409558130905No Hit
ATCATGGTCATGGTCTCTGACGGCTCTA92770.10267480199656392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)