FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004724495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8651925
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCTAGAGTACCGTCATCCTCGTTT1843592.1308437139711685No Hit
AACCGCGGGAACCCCGCATCTTCACGGG1613901.8653652221904373No Hit
TGGCTGGAGCTGATAATAGCCCTAACTT1488961.7209580526876969No Hit
CACCTCAGCAATATGATCTAGTGGATTT1267231.4646798255879472No Hit
GTCCTCAAGGTCATCTCTCACACGCATT1256631.4524282168419167No Hit
GCAAACTTCATCGCTCACTTTGTCCATT1202401.3897485241723664No Hit
GACGGCTCTATGAAATCTTATCTCGAGG1002231.1583896069371844No Hit
CAGCTAAAGCCGGTAATATAATGAACTT996901.1522291281997938No Hit
TGGCTGGAGCTGATAACTAGTGACATTT977451.1297485819629736No Hit
AGTGGGAAGAGGTAGACGTGGTCAAATT949921.0979290735876697No Hit
ATAACGCGTTCCACTCCCCGAAGGCGTT948611.0964149596766037No Hit
GATCGTAGTGAGGGAGTAGTACGGGATT921921.0655663335038157No Hit
CAAGAAATCGACAGCCAAACCAGTAGTT879131.0161091317828113No Hit
TGGCTGGAGCTGATAAACATAGGCGGTT783180.9052089563883182No Hit
TCATTACCACGTAAGGGTGTGTTACGTT764180.8832485256171314No Hit
CCTCAGTAGCGTTGCCAAGATGTTCTTT752340.8695637098102446No Hit
TGGCTGGAGCTGATAAAACTTTCCTTTT710930.8217015288505161No Hit
TCTGAGATCTGCGTAACCTTCTGCCGTT706310.81636167673668No Hit
CAGCTGGAGCCCGAAACGTCAGCCTTTT697190.8058206699665104No Hit
AGTTGGTAGCCGGTAAAATGAAGTATTT695810.8042256492052346No Hit
AGCTCCTAGAGTACCGCGACAAAACCTT674830.7799767103852611No Hit
TCTGAGACAACTGCTAAAGTCTTATCTT638860.738402147498967No Hit
GTTCATTTCGTCCAGGTACATTGTAATT626560.7241856581049883No Hit
GCTGGGTAGTAACCCTGTCTATCAGGTT588570.6802763546840732No Hit
CGTAGCGAGTTCGCATTACATTGGACTT577380.66734281677199No Hit
TGGCTGGAGCTGATAACTCATTAGCTTT570310.6591712249008169No Hit
CAGCTGGTCTGCCAGGGAAGTTCGGGTT503800.5822981590802047No Hit
GGCGACTAGGACATTACGGGAGGTGGTT490090.5664519745605747No Hit
CAGCAGCGGCCTTTGTTTTCAGACCCTT481140.5561074558551999No Hit
TACTCGCTCTTAACCTTGTTATGCGTTT470830.5441910326314664No Hit
ATGTGTGGTACGAAATGACCACGCCGTT464650.5370481135701014No Hit
TCTGAGACAACTGCTAATTTTTACTGTT440130.508707599753812No Hit
CATATTCAGTTTCCTTCCTGTCGTCCTT436450.5044542110570769No Hit
ATTGGTGTCCAGTAGTCAACGGTCATTT420310.4857994030230267No Hit
GGACAGATCGGTTAACGAGTGGAATCTT391280.45224617642894493No Hit
TAGGCATTCTGTTGAGAGGCGTGCCGTT372530.4305746986942212No Hit
TGGCTGGAGCTGATAAAAACATTGGGTT352190.4070654796475929No Hit
TACCTTAAGATGTGTAGGCATGCACTTT325870.37664450396877No Hit
CTACATTAGAATGTGTCCGGGGCCAGTT324460.3750148088431187No Hit
CGTCCATACATAGCTACCCTGCTGCGCT317600.36708593752257446No Hit
CGATGGCGTTTACTCTGGGTGTATCGTT316960.3663462177492292No Hit
TAGGCATTCTGTTGAGGTGGGAGGCGTT296220.3423746738442601No Hit
TAGACCATCTCTTATGTTCCATCCGGTT275940.31893480352638287No Hit
AGTGTCATCCTTAATCACGGTCCGCTTT272030.3144155780361018No Hit
CTACATTAGAATGTGTAAAAAGGCCGTT247260.28578611118334935No Hit
ATCCTACTTCTAACTCTGAGTCACCGTT238170.2752797787775553No Hit
ATCTACTGTACAAGTAATTTCGCTACTT230650.26658807144074875No Hit
AGAATAGAGACTAGATTCGCCGCCTATT226430.2617105441852536No Hit
TCTGAGAAGGATGTATGAACTCCTGATT206890.2391259748553068No Hit
TGGCTGGAGCTGATAATAGCCACGGCTT202030.23350872782646634No Hit
GAACATCAGGAATTACGTCGACTCGTTT182050.2104156011523447No Hit
TCTGAGAAGGATGTATCCGTCTCGGCTT181700.21001106690129653No Hit
ATCTACTTCTAACTCTTCTCTCCCTGTT178710.20655518858519925No Hit
ATTACTCGTGTCAATCCGGGGGCTTCTT177190.2047983541235043No Hit
GCGGGTTTCGAATCCACCTGGCAAAATT174310.20146961514345071No Hit
GAACATCAGGAATTACATATTAACGGTT162310.1875998693932275No Hit
TGACGGCTCTATGAAATCTTATCTCGAG158970.18373945682608206No Hit
TACTTGTAGATGTGGCTTACTAGGTTTT158590.18330024821065832No Hit
CCACTACCCATTACCAGATCGCTTCACA155970.18027202038852624No Hit
TACACGAGTAATAGCAGAGTTTCTTTTT149790.1731291013271613No Hit
AAGGTTCGTGTTGGGATGTAACGTAGTT146480.1693033631243914No Hit
TCTCAAGTCTCTCTGTAGAGCGGGCTTT144810.16737315684081866No Hit
GACTGCGGGAACCCCGCATCTTCACGGG143400.16574346171516743No Hit
TCATTACTCCCAAGTAGTGTTTAGACTT129090.14920378990802627No Hit
CTCGAGGGAGGCTTCCCGCTTAGATGCT127570.14744695544633132No Hit
ACATCAGTCACTTATCAAATCAAGCCTT125040.14452275071732593No Hit
AGTGGGTATAATACTAAGTTGAGATGAT124620.1440373096160681No Hit
TATTACCGTAAGTAGTCAAAAGTTGCTT122280.14133270919477456No Hit
TTCAACGATCTAGCACATGCGTTGTGGT119990.13868589938077364No Hit
TTGGAACCAGGGATTGGGTCTATCGCTT119810.13847785319452027No Hit
TATTACCGTAAGTAGTGAAGTTCTTGTT113260.1309072836391901No Hit
ACTGATGCAATGAAACGCTCCATCCTTT112550.13008665701563524No Hit
GCTTGAATCTTTAGTCTAACCTATGATT111870.12930070475645594No Hit
AAGCCGCGGGAACCCCGCATCTTCACGG102510.11848230307128184No Hit
TTCTCAAGTCATACTGCATGTTATGGTT102290.1182280243991944No Hit
TAGGCATTCTGTTGAGGTTCTTCAATTT100330.11596263259332461No Hit
AACCATGGGAACCCCGCATCTTCACGGG99410.11489928541914082No Hit
TCTGAGAAGGATGTATCGTATTTATCTT98240.11354698520849406No Hit
TAGGCATTCTGTTGAGCGCTACCCAGTT91900.1062191362037928No Hit
GGGCATCATTTCAGAGCTTGCACCCCTC91350.10558343952357424No Hit
ATCGAGTCATGGTCTCTGACGGCTCTAT90540.10464723168543417No Hit
ATCATGGTCATGGTCTCTGACGGCTCTA87330.10093707469724945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)