FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004724501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056913
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC107250.5214124272635741No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTG97910.47600457578905864No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC70570.34308694631226505No Hit
CAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGC59140.2875182372808184No Hit
CAGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCT44270.2152254373422697No Hit
ACGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGC43590.21191951239551698No Hit
CAGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA41360.2010780232319014No Hit
CGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATA38710.18819463924823265No Hit
ATGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAAT34060.16558794659764414No Hit
GCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATA33460.1626709539975682No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT29560.1437105020970746No Hit
ACGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGT29160.14176584036369064No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA27730.13481367466684296No Hit
AGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA25460.12377771932988901No Hit
GTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA24490.11906191462643292No Hit
CCCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGT22140.10763702694280214No Hit
GCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTC21390.10399078619270723No Hit
CAGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGT21220.10316430495601904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)