FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004724511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587842
Sequences flagged as poor quality0
Sequence length28
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGGAAGGCTATCTATGTCTCGGCTT17226610.84906432755904No Hit
TGCGGGTAGGTAGCCACCCGCGTGCGTT1141267.1874909468322405No Hit
GGGCATCGTTCTGAACGGCTTGGTTCTT891575.614979324139304No Hit
CACATAGCAATAGCGGTTTTACCCTTTT709864.470595940905959No Hit
TATCTCAGTCGTTGTATCTCCGCCTCTT158420.9977063209059843No Hit
ATCCGAATCGATCCCTTCTCAAACTCTT143890.9061984756669744No Hit
GAACATCCACCCATTCTGCCTGGTAGTT137230.8642547558258316No Hit
GGCTCGATCATAACCGCCGCTATGGTTT129990.8186582796021266No Hit
GCTTGAAGTAGCCTATCTCACGTATCTT115040.7245053349136753No Hit
AGCTCTCCAGGGTTAGGTAAAACTCCTT112430.7080679311921463No Hit
GAACATCCACCCATTCTTTTGCCTCCTT107620.6777752446402098No Hit
CCGTACTAGCTCAACTCCTTAGTCGCTT105200.6625344335267614No Hit
CATGCCTTCTCGCATCGTTTTTGCCTTT103560.6522059499622759No Hit
CAGCTAACAGCTCGACTCGGCGTCCTTT98930.6230468774600999No Hit
CATGCCTTCTCGCATCTTGTTCTCTCTT94530.5953363117992848No Hit
GGGCACTCAAGTTCTGTGGCCGAGCCTT90130.5676257461384696No Hit
TTGGAACCAATAACGATGCTAGCCCGTT89240.5620206544479867No Hit
GGGCATCGTTCTGAACCGTCTGCGTATT88400.5567304555491037No Hit
AGTGGGAAGGCTATCTTCCATGCCAGTT80300.5057178233098759No Hit
TGCACCTTCTCATTCAAAACGTGCACTT78430.4939408329040295No Hit
CCGTACTAGCTCAACTTTAAGTCGGGTT78280.49299615452922896No Hit
GTACGTAGTCATGCCGGACAATGGTCTT77900.4906029693130677No Hit
CCGTACTAGCTCAACTTGAATAACTCTT77420.48757999851370604No Hit
GGGCATCGTTCTGAACATGCCAGGGTTT74860.4714574875837772No Hit
AGCTCTCCAGGGTTAGATCAGCAGCGTT74300.46793068831785534No Hit
CATGCCTTCTCGCATCACTCCGGGGCTT73870.4652226103100938No Hit
TGCGGGTAGGTAGCCACGATGTCCTCTT73450.46257751086065235No Hit
ATCCGAATCGATCCCTGCCGCAACCTTT70840.44614010713912344No Hit
TGCACCTTCACTGGGCGTCGTTCTGTTT69220.43593758069127786No Hit
CACATAGCAATAGCGGCAAACCTCCTTT68290.4300805747675146No Hit
GGCTCGATCATAACCGTAGAATGGGCTT67730.42655377550159274No Hit
TCGCGAGCGCTCTGGCTTTTTCTTATAT66510.4188703913865485No Hit
TTTCCTCAGCGTTTACCCCGTGTGCGTT66300.4175478416618278No Hit
GATGCTAAGGCATTGGCTATGAACCATT62290.392293439775494No Hit
GGGCATCGTTCTGAACCGCTGGCTCATT62130.3912857828423735No Hit
CGATGGCCATCCCACTACATATCTCGTT60800.38290963458580896No Hit
ATCCGAATCGATCCCTAGGCTTTCCATT59130.3723922153463632No Hit
TGCGGGTAGGTAGCCAACCGACTCGTTT58630.36924328743036144No Hit
GTACGTAGTCATGCCGTTGGTCTCCCTT57510.3621896888985176No Hit
GATGCTAAGGCATTGGATCCATGCGGTT57220.36036331070723665No Hit
AACTCAGTCCAGAAGGTAATTCCGCCTT57130.35979650368235633No Hit
ATCACGAAGCCAGTAGTGAGACCGTGTT55450.3492161058845905No Hit
GGGCACTCAAGTTCTGGCCGTTCTGTTT55090.3469488777850693No Hit
CTTTGCGCAGTTAACCATGTGGAGGCTT54960.3461301565269088No Hit
GGCTCGATCATAACCGGGCCGACAACTT54580.34373697131074754No Hit
CCGTACTAGCTCAACTCATGCACTACTT54470.3430442071692272No Hit
ATCCACCCAATCCGATGCAGGGGGGGTT54430.34279229293594704No Hit
ATCCACCCAATCCGATACGATGGGCTTT54000.3400842149281855No Hit
GGGCATCGTTCTGAACTCTATATGCCTT53080.3342901875627424No Hit
AGCTCTCCAGGGTTAGCCTGGCTTGTTT51020.32131660454881533No Hit
GGCTCGATCATAACCGGCCCGACCATTT51000.32119064743217524No Hit
AACTCAGTCCAGAAGGATGGGGGCCGTT50120.3156485343000122No Hit
GGGCATCGTTCTGAACCGGACCCGTCTT50010.31495577015849185No Hit
CCGTACTAGCTCAACTTCATCGATAGTT49090.30916174279304864No Hit
GTTAAGCTCTGTCCGTCCACTGCCCCTT48360.3045643080356862No Hit
CCGTACTAGCTCAACTCCCGCGCTCATT47890.30160431579464453No Hit
GGGCATCGTTCTGAACGAGGTTTCCATT47760.30078559453648407No Hit
GGGCACTCAAGTTCTGACCAAGCCGGTT47550.2994630448117634No Hit
CACATAGCAATAGCGGGGTAGTTCCTTT47360.29826645220368275No Hit
ATCCGAATCGATCCCTCGTTAAGTGCTT46430.29240944627991955No Hit
GTACGTAGTCATGCCGGGACGGAGCTTT46350.2919056178133593No Hit
CTTTGCGCAGTTAACCTCACAGGTCCTT46280.291464767905119No Hit
TGCGGGTAGGTAGCCATAGTGACGGGTT46190.2908979608802387No Hit
GCTGCGAAGTACGTAACCGCATCTCTTT45880.2889456255723176No Hit
GCTTGAAGTAGCCTATAGCCTATGGGTT45860.2888196684556776No Hit
CCGTACTAGCTCAACTACGCCTCCATTT45350.28560776198135585No Hit
GGGCATCGTTCTGAACTAACTGTTCCTT45250.2849779763981555No Hit
ATCCACCCAATCCGATATCCCGTCCCTT44440.2798767131742327No Hit
CATGCCTTCTCGCATCCTCCCCTCCATT44240.27861714200783205No Hit
GTTAAGCTCTGTCCGTCCAACGCCTGTT41730.2628095238695034No Hit
AACTCAGTCCAGAAGGTTCGGTTGGCTT41590.2619278240530229No Hit
AACTCAGTCCAGAAGGTAGGGGGCTGTT41090.2587788961370212No Hit
AGTGGGAAGGCTATCTGAGTATAGGTTT40900.2575823035289405No Hit
GGCTCGATCATAACCGCTATGGGTCCTT40800.2569525179457402No Hit
CTTTGCGCAGTTAACCCTGAGCAGCTTT40800.2569525179457402No Hit
GAACATCCACCCATTCTTCGGTTGGTTT40750.25663762515414No Hit
TACTCGCCAGGGTTAGTGTGGTTGGATT40590.25562996822101947No Hit
AGTGGGAAGGCTATCTTCCCTAACACTT40450.254748268404539No Hit
CCGTACTAGCTCAACTCCCAAAGCCCTT38200.24057809278253123No Hit
GCTGCGAAGTACGTAAGTCGAGCCGCTT38140.24020022143261105No Hit
TACTCGCCAGGGTTAGCCCCGCCACTTT37820.23818490756636995No Hit
TACCTTAAGCAGGTCAAATACGATCCTT37270.23472108685876808No Hit
GAACATCCACCCATTCGCACTATTCTTT36040.22697472418540385No Hit
ATCACGAAGCCAGTAGCGGAGCCCCTTT35880.2259670672522833No Hit
TACCTTAAGCAGGTCACATGTCGGGTTT35350.22262920366132147No Hit
GATGCTAAGGCATTGGCGTTACGAGTTT35000.22042495412012025No Hit
TGCACCTTCACTGGGCCTTCCCTCTGTT34920.21992112565356No Hit
ACTGTCCTCGTTGCCTGTGCGCGGGCTT34730.21872453304547934No Hit
ATCACGAAGCCAGTAGGGGAGGCCTCTT34480.21715006908747847No Hit
TGCGGGTAGGTAGCCACGATCCCCCATT33460.21072625613883497No Hit
GGCTCGATCATAACCGAGTCTTGGGGTT33440.21060029902219493No Hit
TACTCGCCAGGGTTAGCGATGGGGGGTT31940.20115351527418973No Hit
GGCTCGATCATAACCGTTGGTCGGACTT31910.20096457959922964No Hit
AAACGGGTCTTATCTGGGCCCCCGTTTT31790.20020883689938923No Hit
GGGCATCGTTCTGAACACCTGTGTTATT31660.19939011564122877No Hit
GAACATCCACCCATTCGCGTGGCATCTT31360.19750075889162777No Hit
TGCGGGTAGGTAGCCAACCTCGCCCCTT31220.1966190590751473No Hit
ATCACGAAGCCAGTAGGTCCAGTCCCTT30700.1933441740425055No Hit
GAACATCCACCCATTCGTTATTTGGTTT30080.18943950342666335No Hit
GGGCACTCAAGTTCTGCCCTCCCTCCTT30040.18918758919338322No Hit
CTTTGCGCAGTTAACCCTCTACACCTTT29930.18849482505186285No Hit
GGGCATCGTTCTGAACTGGATCAGCGTT29860.1880539751436226No Hit
GGGCACTCAAGTTCTGTCGTGTGGGTTT29330.18471611155266077No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATA28970.18244888345313953No Hit
AACTCAGTCCAGAAGGTTACGTGCTATT28780.1812522908450589No Hit
TACTCGCCAGGGTTAGTGCTTTCTGTTT28310.1782922986040173No Hit
AGCTCTCCAGGGTTAGCTTTGGTTGGTT27680.1743246494298551No Hit
TACTCGCCAGGGTTAGCCATCGGTGCTT27600.17382082096329485No Hit
GGGCATCGTTCTGAACAGACTGCGCTTT27240.17155359286377359No Hit
TGCGGGTAGGTAGCCAATTATGCGCGTT27040.17029402169737293No Hit
TACCTTAAGCAGGTCAGTGCGAACGCTT26930.16960125755585254No Hit
GTACGTAGTCATGCCGAACAAAGCCGTT26910.16947530043921247No Hit
TCGCGAGAGTAAGTACTATGTGACACTT26420.16638935108153077No Hit
GCTGCGAAGTACGTAATCCATACGGGTT26250.1653187155900902No Hit
CCGTACTAGCTCAACTTGATGTCGCATT25820.16261063758232872No Hit
CCGTACTAGCTCAACTATGTCCTTCATT25810.1625476590240087No Hit
ATCCACCCAATCCGATAGGCCTAGAGTT25510.16065830227440767No Hit
TGGTTCCTCGATGAGGTCATGGGGGGTT25190.15864298840816657No Hit
ACCTTTATTCTAATGTGAACCATGGCCC24690.15549406049216485No Hit
GCTGCGAAGTACGTAACGTGTGCTGTTT24690.15549406049216485No Hit
GGGCATCGTTCTGAACTGCCGTCATCTT24520.15442342500072426No Hit
ATCCACCCAATCCGATCACCATGGTCTT24450.15398257509248403No Hit
TGCGGGTAGGTAGCCACTCGGCCGCTTT23700.14925918321848142No Hit
ATCCACCCAATCCGATACCTTCTGGGTT23460.14774769781880062No Hit
GCTTGAAGTAGCCTATCCATTCAGATTT22770.14340217729471824No Hit
ATCACGAAGCCAGTAGCGTGAGTCCCTT22050.13886772109567577No Hit
TAGTTGGGTATAAACGGTTAGATCCTTT21870.13773410704591516No Hit
TGCACCTTCACTGGGCTGGTTATCAATT21740.13691538578775472No Hit
ATCCACCCAATCCGATCACATGGGTGTT21460.13515198615479374No Hit
CCGTACTAGCTCAACTTGAGTACCCTTT20870.1314362512139117No Hit
GGGCATCGTTCTGAACGCAGCGCTACTT20870.1314362512139117No Hit
GGGCATCGTTCTGAACCATCGCGTCCTT20850.13131029409727163No Hit
TCGCGAGAGTAAGTACACCCTCAGGATT20660.130113701489191No Hit
GGGCATCGTTCTGAACGTTGGGGCTTTT20430.1286651946478302No Hit
TGCACCTTCACTGGGCAACGCACCCTTT20250.12753158059806957No Hit
TACTCGCCAGGGTTAGTCCGATTTGCTT19900.12532733105686836No Hit
TGCGGGTAGGTAGCCACCTGCGCCTGTT19760.12444563124038789No Hit
TGGTTCCTCGATGAGGATATCTATAATT18350.11556565451726304No Hit
AGTGGGAAGGCTATCTCGCTTGTTCTTT18060.11373927632598205No Hit
GTACGTAGTCATGCCGACTTCTTTCATT17150.10800822751885894No Hit
GATGCTAAGGCATTGGCATTCCTCGATT17000.10706354914405841No Hit
AGTGGGAAGGCTATCTACGACGGCCGTT16850.1061188707692579No Hit
GCTTGAAGTAGCCTATGTCCTCTCCATT16820.1059299350942978No Hit
TATCTCAGTCGTTGTAATTTCTTCTCTT16770.10561504230269761No Hit
GGGCATCGTTCTGAACCCCGAACCCTTT16680.10504823527781731No Hit
TACCTTAAGCAGGTCATTCTATAGAGTT16450.10359972843645651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)