FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004724512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587842
Sequences flagged as poor quality0
Sequence length89
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1015146.393205369299968No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC711544.481176338703725No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC470162.9609998979747356No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC168821.06320402155882No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG165101.0397759978637673No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG148510.9352945696108302No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG137010.8628692275427908No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG127340.8019689616473177No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC117900.7425172025932052No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC92170.5804733720357568No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTG90370.5691372315381504No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT86180.5427492156020561No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTG85300.5372071024698931No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGG84190.5302164824963693No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCA76060.4790149145821814No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT62310.3924193968921341No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT60270.37957177099484707No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC54440.3428552714942671No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTG54120.34083995762802594No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTAAGATCC53570.33737613692042406No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG51250.3227651113901761No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC46930.2955583741959213No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA40180.2530478473298981No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTC39780.2505287049970967No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGAGAG37600.23679937928332923No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGGAGA36190.22791940256020435No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTC32620.2054360572399521No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA31300.19712288754170754No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACT29850.18799099658530258No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA27150.1709867858388933No Hit
GGTCTCCAGACCTAGGGGAGCATCTCAGCGTCACTCGCTGTCCAGTTGCT26300.16563360838169036No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25110.15813915994160627Clontech SMART CDS Primer II A (100% over 26bp)
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGA24130.1519672612262429No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATC22810.14365409152799838No Hit
GTTCAAGAATGTTTCCTTTTGCTCTCTCTTCTCCATTATAATACTGAATG22690.14289834882815797No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGA22430.14126090631183708No Hit
GTTCAGAGTGCAAGTCAGGGAACTGTTGTGCGGAGAATCGTTCAAGAATG22330.14063112072863673No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA22030.1387417639790357No Hit
CTCCAGACCTAGGGGAGCATCTCAGCGTCACTCGCTGTCCAGTTGCTGTG21530.135592836063034No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG21000.13225497247207216No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG20500.12910604455607044No Hit
GTTGGTACCAGTACACAGAGAGGTCTCCAGACCTAGGGGAGCATCTCAGC19660.12381584565718756No Hit
GGTACCAGTACACAGAGAGGTCTCCAGACCTAGGGGAGCATCTCAGCGTC19640.12368988854054748No Hit
GGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGGTCTCCAGAC19240.12117074620774612No Hit
GGAACTGGAGGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGG18890.11896649666654491No Hit
GGTTTGTGTGACTCCAGAATCCACTGGGCCTGCTCCCAGGAGACAAAAGG18570.11695118280030381No Hit
GCATCTCAGCGTCACTCGCTGTCCAGTTGCTGTGATCAGGTGCTTTGGGG18100.11399119055926218No Hit
GGTGCTTTGGGGTTTGTGTGACTCCAGAATCCACTGGGCCTGCTCCCAGG18030.11355034065102196No Hit
GGAGGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGGTCTCCA16810.10586695653597776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16270.1024661143866959No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC16190.10196228592013563No Hit
GGAGCATCTCAGCGTCACTCGCTGTCCAGTTGCTGTGATCAGGTGCTTTG15990.10070271475373495No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGT15950.1004508005204548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)