FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004724513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598566
Sequences flagged as poor quality0
Sequence length28
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGGAAGGCTATCTATGTCTCGGCTT17162310.7360596935003No Hit
TGCGGGTAGGTAGCCACCCGCGTGCGTT1147487.178183446914296No Hit
GGGCATCGTTCTGAACGGCTTGGTTCTT908255.681654683009647No Hit
CACATAGCAATAGCGGTTTTACCCTTTT712644.457995478447558No Hit
TATCTCAGTCGTTGTATCTCCGCCTCTT157350.9843196965280132No Hit
ATCCGAATCGATCCCTTCTCAAACTCTT147010.9196367244142563No Hit
GAACATCCACCCATTCTGCCTGGTAGTT137320.8590198965823119No Hit
GGCTCGATCATAACCGCCGCTATGGTTT135200.8457580106170155No Hit
GCTTGAAGTAGCCTATCTCACGTATCTT117100.7325315313850038No Hit
AGCTCTCCAGGGTTAGGTAAAACTCCTT115810.7244617988872527No Hit
CATGCCTTCTCGCATCGTTTTTGCCTTT111690.6986886997471484No Hit
GAACATCCACCCATTCTTTTGCCTCCTT110940.6939969948065954No Hit
CCGTACTAGCTCAACTCCTTAGTCGCTT107050.669662685181594No Hit
CAGCTAACAGCTCGACTCGGCGTCCTTT102330.6401362220890473No Hit
CATGCCTTCTCGCATCTTGTTCTCTCTT97620.6106723150623746No Hit
GGGCACTCAAGTTCTGTGGCCGAGCCTT91710.5737016801308172No Hit
TTGGAACCAATAACGATGCTAGCCCGTT91680.5735140119331951No Hit
GGGCATCGTTCTGAACCGTCTGCGTATT89150.5576873272670632No Hit
AGTGGGAAGGCTATCTTCCATGCCAGTT79660.4983216207525995No Hit
CCGTACTAGCTCAACTTTAAGTCGGGTT78980.4940678082731648No Hit
CATGCCTTCTCGCATCACTCCGGGGCTT78510.49112767317708494No Hit
GGGCATCGTTCTGAACATGCCAGGGTTT78300.4898139957937302No Hit
CCGTACTAGCTCAACTTGAATAACTCTT77790.48662363643415407No Hit
AGCTCTCCAGGGTTAGATCAGCAGCGTT76090.4759891052355674No Hit
GTACGTAGTCATGCCGGACAATGGTCTT75290.47098461996564417No Hit
TGCACCTTCTCATTCAAAACGTGCACTT73760.4614135418869162No Hit
TGCGGGTAGGTAGCCACGATGTCCTCTT73130.45747250973685166No Hit
TGCACCTTCACTGGGCGTCGTTCTGTTT71350.4463375300112726No Hit
GGCTCGATCATAACCGTAGAATGGGCTT70820.44302205851994847No Hit
CACATAGCAATAGCGGCAAACCTCCTTT70480.44089515228023113No Hit
ATCCGAATCGATCCCTGCCGCAACCTTT68170.426444701063328No Hit
TCGCGAGCGCTCTGGCTTTTTCTTATAT67390.4215653279251529No Hit
TTTCCTCAGCGTTTACCCCGTGTGCGTT65570.4101801239360777No Hit
GGGCATCGTTCTGAACCGCTGGCTCATT63170.3951666681263082No Hit
GATGCTAAGGCATTGGCTATGAACCATT63000.3941032150064495No Hit
CGATGGCCATCCCACTACATATCTCGTT61650.3856581461134542No Hit
AACTCAGTCCAGAAGGTAATTCCGCCTT60380.37771352574745115No Hit
ATCCGAATCGATCCCTAGGCTTTCCATT58720.3673292188123606No Hit
TGCGGGTAGGTAGCCAACCGACTCGTTT58430.36551509290201345No Hit
ATCACGAAGCCAGTAGTGAGACCGTGTT57390.3590092620511133No Hit
GATGCTAAGGCATTGGATCCATGCGGTT56830.3555061223621671No Hit
GTACGTAGTCATGCCGTTGGTCTCCCTT56380.35269109939783533No Hit
CTTTGCGCAGTTAACCATGTGGAGGCTT55790.34900029151126694No Hit
GGCTCGATCATAACCGGGCCGACAACTT55160.34505925936120246No Hit
GGGCACTCAAGTTCTGGCCGTTCTGTTT55130.34487159116358035No Hit
CCGTACTAGCTCAACTCATGCACTACTT54900.34343280164847745No Hit
ATCCACCCAATCCGATGCAGGGGGGGTT54300.3396794376960351No Hit
ATCCACCCAATCCGATACGATGGGCTTT52820.3304211399466772No Hit
GGCTCGATCATAACCGGCCCGACCATTT52780.330170915683181No Hit
AGCTCTCCAGGGTTAGCCTGGCTTGTTT50980.3189108238258539No Hit
GGGCATCGTTCTGAACTCTATATGCCTT50380.31515745987341154No Hit
GGGCATCGTTCTGAACCGGACCCGTCTT50350.3149697916757894No Hit
AACTCAGTCCAGAAGGATGGGGGCCGTT50120.3135310021606865No Hit
CCGTACTAGCTCAACTTCATCGATAGTT49110.3072128395074085No Hit
GTTAAGCTCTGTCCGTCCACTGCCCCTT48850.30558638179468345No Hit
GCTGCGAAGTACGTAACCGCATCTCTTT48370.3025836906327296No Hit
GGGCATCGTTCTGAACGAGGTTTCCATT48160.30127001324937475No Hit
GGGCATCGTTCTGAACTAACTGTTCCTT47920.2997686676683978No Hit
ATCCGAATCGATCCCTCGTTAAGTGCTT47790.2989554388120353No Hit
CTTTGCGCAGTTAACCTCACAGGTCCTT47780.2988928827461612No Hit
CCGTACTAGCTCAACTCCCGCGCTCATT47660.29814220995567275No Hit
GTACGTAGTCATGCCGGGACGGAGCTTT47660.29814220995567275No Hit
GGGCACTCAAGTTCTGACCAAGCCGGTT46980.2938883974762381No Hit
TGCGGGTAGGTAGCCATAGTGACGGGTT46850.29307516861987554No Hit
CACATAGCAATAGCGGGGTAGTTCCTTT46000.28775790302058224No Hit
ATCCACCCAATCCGATATCCCGTCCCTT45480.28450498759513215No Hit
CCGTACTAGCTCAACTACGCCTCCATTT45290.2833164223435254No Hit
GCTTGAAGTAGCCTATAGCCTATGGGTT44890.2808141797085638No Hit
CATGCCTTCTCGCATCCTCCCCTCCATT43940.27487135345053004No Hit
GGCTCGATCATAACCGCTATGGGTCCTT42890.2683029665337559No Hit
AACTCAGTCCAGAAGGTTCGGTTGGCTT41730.26104646289236727No Hit
AACTCAGTCCAGAAGGTAGGGGGCTGTT41660.260608570431249No Hit
AGTGGGAAGGCTATCTGAGTATAGGTTT41250.2580437717304134No Hit
CTTTGCGCAGTTAACCCTGAGCAGCTTT40580.2538525153168527No Hit
AGTGGGAAGGCTATCTTCCCTAACACTT40010.25028681956203247No Hit
TACTCGCCAGGGTTAGTGTGGTTGGATT39710.24841013758581126No Hit
GTTAAGCTCTGTCCGTCCAACGCCTGTT39620.24784713299294492No Hit
GCTGCGAAGTACGTAAGTCGAGCCGCTT38870.24315542805239196No Hit
TACTCGCCAGGGTTAGCCCCGCCACTTT38270.23940206409994957No Hit
TACCTTAAGCAGGTCAAATACGATCCTT38200.2389641716388313No Hit
GAACATCCACCCATTCTTCGGTTGGTTT37710.23589892441100335No Hit
GAACATCCACCCATTCGCACTATTCTTT36740.22983098602122154No Hit
CCGTACTAGCTCAACTCCCAAAGCCCTT36540.2285798647037407No Hit
ATCACGAAGCCAGTAGCGGAGCCCCTTT36530.2285173086378667No Hit
TACCTTAAGCAGGTCACATGTCGGGTTT36350.227391299452134No Hit
ATCACGAAGCCAGTAGGGGAGGCCTCTT35500.2220740338528406No Hit
TGCACCTTCACTGGGCCTTCCCTCTGTT34190.2138791892233414No Hit
GATGCTAAGGCATTGGCGTTACGAGTTT33430.20912492821691442No Hit
ACTGTCCTCGTTGCCTGTGCGCGGGCTT33350.20862447968992207No Hit
GGCTCGATCATAACCGAGTCTTGGGGTT33200.20768613870181152No Hit
GGCTCGATCATAACCGTTGGTCGGACTT33150.20737335837244128No Hit
TGCGGGTAGGTAGCCACGATCCCCCATT32890.20574690065971626No Hit
GAACATCCACCCATTCGTTATTTGGTTT32110.2008675275215412No Hit
TACTCGCCAGGGTTAGCGATGGGGGGTT31720.1984278409524536No Hit
ATCACGAAGCCAGTAGGTCCAGTCCCTT31640.1979273924254613No Hit
TGCGGGTAGGTAGCCAACCTCGCCCCTT31430.19661371504210648No Hit
GGGCATCGTTCTGAACACCTGTGTTATT30990.19386124814364875No Hit
AAACGGGTCTTATCTGGGCCCCCGTTTT30980.1937986920777747No Hit
GAACATCCACCCATTCGCGTGGCATCTT30230.18910698713722174No Hit
GGGCATCGTTCTGAACTGGATCAGCGTT29370.18372716547205434No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATA29150.18235093202282546No Hit
GGGCACTCAAGTTCTGCCCTCCCTCCTT29080.18191303956170718No Hit
GGGCACTCAAGTTCTGTCGTGTGGGTTT29040.18166281529821104No Hit
CTTTGCGCAGTTAACCCTCTACACCTTT28570.17872268020213117No Hit
TACCTTAAGCAGGTCAGTGCGAACGCTT27770.173718194932208No Hit
GGGCATCGTTCTGAACAGACTGCGCTTT27680.17315519033934162No Hit
AGCTCTCCAGGGTTAGCTTTGGTTGGTT27560.17240451754885316No Hit
AACTCAGTCCAGAAGGTTACGTGCTATT27510.17209173721948295No Hit
TACTCGCCAGGGTTAGCCATCGGTGCTT27210.17021505524326178No Hit
TGCGGGTAGGTAGCCAATTATGCGCGTT26890.1682132611352925No Hit
TACTCGCCAGGGTTAGTGCTTTCTGTTT26750.16733747621305595No Hit
GCTGCGAAGTACGTAATCCATACGGGTT26650.16671191555431555No Hit
TGGTTCCTCGATGAGGTCATGGGGGGTT26630.1665868034225675No Hit
ATCCACCCAATCCGATAGGCCTAGAGTT26220.16402200472173187No Hit
CCGTACTAGCTCAACTATGTCCTTCATT26120.16339644406299147No Hit
TCGCGAGAGTAAGTACTATGTGACACTT26080.16314621979949528No Hit
CCGTACTAGCTCAACTTGATGTCGCATT25580.16001841650579332No Hit
GTACGTAGTCATGCCGAACAAAGCCGTT25490.15945541191292695No Hit
ATCCACCCAATCCGATCACCATGGTCTT25270.1580791784636981No Hit
GGGCATCGTTCTGAACTGCCGTCATCTT25220.15776639813432788No Hit
GCTGCGAAGTACGTAACGTGTGCTGTTT24510.1533249174572711No Hit
ACCTTTATTCTAATGTGAACCATGGCCC24220.15151079154692393No Hit
TGCGGGTAGGTAGCCACTCGGCCGCTTT23420.14650630627700076No Hit
ATCCACCCAATCCGATACCTTCTGGGTT23040.14412917577378725No Hit
GCTTGAAGTAGCCTATCCATTCAGATTT22460.14050092395309297No Hit
ATCACGAAGCCAGTAGCGTGAGTCCCTT22250.13918724656973813No Hit
CCGTACTAGCTCAACTTGAGTACCCTTT22020.1377484570546352No Hit
TGCACCTTCACTGGGCTGGTTATCAATT22010.13768590098876118No Hit
ATCCACCCAATCCGATCACATGGGTGTT21820.13649733573715442No Hit
TAGTTGGGTATAAACGGTTAGATCCTTT21480.13437042949743708No Hit
GGGCATCGTTCTGAACGCAGCGCTACTT21370.13368231277282264No Hit
TCGCGAGAGTAAGTACACCCTCAGGATT21030.1315554065331053No Hit
TGCACCTTCACTGGGCAACGCACCCTTT20700.12949105635926197No Hit
GGGCATCGTTCTGAACGTTGGGGCTTTT20510.12830249110765524No Hit
GGGCATCGTTCTGAACCATCGCGTCCTT20430.12780204258066294No Hit
TGGTTCCTCGATGAGGATATCTATAATT19430.12154643599325897No Hit
TACTCGCCAGGGTTAGTCCGATTTGCTT19310.12079576320277048No Hit
TGCGGGTAGGTAGCCACCTGCGCCTGTT18550.11604150219634347No Hit
AGTGGGAAGGCTATCTCGCTTGTTCTTT18200.11385203989075209No Hit
GTACGTAGTCATGCCGACTTCTTTCATT17410.10891011068670296No Hit
TATCTCAGTCGTTGTAATTTCTTCTCTT17040.10659553624936349No Hit
GCTTGAAGTAGCCTATGTCCTCTCCATT16200.10134082671594416No Hit
GGGCATCGTTCTGAACCCCGAACCCTTT16120.10084037818895186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)