FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004724514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598566
Sequences flagged as poor quality0
Sequence length89
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC1041356.514275919793115No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC734984.597745729610163No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC492103.078384001661489No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC175061.0951064891909374No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG168911.056634508678403No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG163061.0200392101420899No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG146320.9153203558689476No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG127720.7989660733432338No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC121280.7586799669203523No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC95290.5960967517137234No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTG95040.5945328500668724No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGACTG90170.5640680459862152No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGG89620.560627462363143No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT85620.5356050360135272No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCA80850.5057657925916103No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT63000.3941032150064495No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT61060.38196733822688583No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTG57100.35719513614076615No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTAAGATCC55820.34918795970888905No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC55190.3452469275588246No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG52860.33067136421017335No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC49540.3099027503399922No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTAAA41020.2566049822153105No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGAGAG40890.255791753358948No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTC39250.24553255855560546No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGGAGA37950.23740026999198033No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGCTTC34410.2152554226725703No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA33570.21000071313915097No Hit
CTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACT31080.1944242527365151No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGA28060.17553232084255513No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26100.16327133193124338Clontech SMART CDS Primer II A (100% over 26bp)
GGTCTCCAGACCTAGGGGAGCATCTCAGCGTCACTCGCTGTCCAGTTGCT25590.16008097257166734No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGA24240.151635903678672No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCAATC23680.1481327639897258No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGA23260.14550540922301614No Hit
GTTCAGAGTGCAAGTCAGGGAACTGTTGTGCGGAGAATCGTTCAAGAATG22920.14337850298329877No Hit
GTTCAAGAATGTTTCCTTTTGCTCTCTCTTCTCCATTATAATACTGAATG22530.14093881641421124No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG21640.1353713265514217No Hit
CTCCAGACCTAGGGGAGCATCTCAGCGTCACTCGCTGTCCAGTTGCTGTG21470.13430787343156303No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGA21430.1340576491680669No Hit
GTTGGTACCAGTACACAGAGAGGTCTCCAGACCTAGGGGAGCATCTCAGC19620.1227350012448657No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG18830.11779307204081658No Hit
GGTACCAGTACACAGAGAGGTCTCCAGACCTAGGGGAGCATCTCAGCGTC18710.1170423992503281No Hit
GGAACTGGAGGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGG18230.1140397080883742No Hit
GGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGGTCTCCAGAC17460.10922289101607315No Hit
GCATCTCAGCGTCACTCGCTGTCCAGTTGCTGTGATCAGGTGCTTTGGGG17360.10859733035733275No Hit
GGTTTGTGTGACTCCAGAATCCACTGGGCCTGCTCCCAGGAGACAAAAGG16930.10590741952474907No Hit
GGAGGCCCTGGTCCAGGCTCTGTTGGTACCAGTACACAGAGAGGTCTCCA16700.10446863000964615No Hit
GGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGT16400.10259194803342495No Hit
GGTGCTTTGGGGTTTGTGTGACTCCAGAATCCACTGGGCCTGCTCCCAGG16290.10190383130881052No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC16160.10109060245244801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)