FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30196272
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52250.010.72584556-57
CGTATGC56050.09.78683354-55
TCGTATG57800.08.42232154-55
CTCGTAT68800.08.00765452-53
CAATTCG313100.06.81828694-95
CGCAATT355600.06.78469994-95
ACGCAAT382100.06.34522994-95
TCTCGTA88700.06.21102194-95
CACGCAA433950.06.128821494-95
TCACGCA492000.06.115192494-95
ATGCCGT89300.06.089528658-59
GTCACGC507750.06.00033394-95
CCGTCTT102700.05.9192760-61
ATCTCGT101900.05.779311794-95
AGTCACG536100.05.76718494-95
AATTCGG99700.05.69247894-95
AATTCGA118700.05.681542494-95
GCAATTC425250.05.600866394-95
AATTCGT82300.05.56872994-95
AATTCGC79000.05.440641494-95
Produced by FastQC (version 0.11.5)