FastQCFastQC Report
Tue 5 Jul 2022
EGAF00004724662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151052720
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTAT74260674.916208725006739TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCGCGTAT6546880.43341688914969556TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGGAT4394210.29090571821546807TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTTT1770310.11719815439271798TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)