FastQCFastQC Report
Sun 3 Jul 2022
EGAF00004724680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113149056
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTAT13008351.149664916338321TruSeq Adapter, Index 9 (97% over 39bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2995230.2647154210460227No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCGCGTAT1624390.143561957777182TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCAGCAATCTCGTAT1366590.12077785253462477TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)