FastQCFastQC Report
Mon 30 May 2022
EGAF00004724684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099296610
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGTAT20412360.18568564493253553TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGGAT14672280.1334697102358935TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGGGT14536960.13223874128020827TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGGTT12229930.11125232160954267TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)