FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004724726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97852174
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTAT16240181.6596647101575892TruSeq Adapter, Index 1 (97% over 37bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3054890.31219439232898394No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCGCGTAT1838100.1878445746131302TruSeq Adapter, Index 1 (97% over 37bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1616430.1651910155823416No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1078330.11019990215035999Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)