FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004725848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004725848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21771754
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA452450.08.78991694-95
TAGATCG158650.08.72507194-95
AGATCGG508350.08.6407194-95
ACACGTC148600.08.62810594-95
ATCGGAA420800.08.31127294-95
CACACGT182650.08.00758694-95
GCACACG212500.07.96656594-95
CACGTCT149550.07.93823794-95
TCGGAAG410550.07.59345194-95
ACGTCTG133700.07.5651794-95
CGTCTGA109700.07.532048794-95
CGGAAGA403550.07.531008294-95
AAGATCG164750.06.816731594-95
CAGATCG162100.06.29833894-95
AGAGCAC356550.06.23298294-95
AGCACAC304500.06.18338694-95
GAGATCG200600.06.07193394-95
GAGCACA355700.05.81399794-95
GAAGAGC508700.05.46557394-95
AAGAGCA471050.05.24715994-95
Produced by FastQC (version 0.11.5)