FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16117298
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA164050.10178505106749282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAA139400.018.8660431
TCAACCG172650.018.8479921
CTCCTAC201750.018.8355884
TCCTTAC250250.018.8303284
CCTCTGA289550.018.8270724
GACGAAC418800.018.8167576
TTCCACC134800.018.8147092
TCCTCTG290200.018.8144043
TACTCCA171450.018.8133543
CACCATA216450.018.813244
CTACTCC172500.018.805832
CACCTTC164050.018.8038443
GGATACC328950.018.8034926
AGTCCTC291100.018.794521
CACGAAG223250.018.789845
ACACGAA222450.018.7889964
TCAGAGC204550.018.7879731
GAGCTTG439200.018.777284
ACACCAT217500.018.7705233
CCTTACG252400.018.7679125
Produced by FastQC (version 0.11.5)