FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14975907
Sequences flagged as poor quality0
Sequence length31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGTTGATACCACTGCTTCCG165060.11021703059454097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG95150.015.45098925
GTATCAA142000.014.3766131
AACGGTA7400.013.51373718
ACGGTAG7650.013.39891319
TATGCGA17550.012.9633065
TCGTGTA14100.012.76706223
ATATGCG18050.012.7425924
TAGGTCG5600.012.7235525
GACCGTC10250.012.6815267
GGTATCA64150.012.2653171
AACGTTG15150.011.88213722
TGCTTCC123100.011.76002624
TGGACTG34050.011.41733555
GGACTGA28000.011.3826336
CGGTAGT9150.011.33924920
CTCGTGT15950.011.20779222
ACCGTCT12000.011.1450118
GCGAATC21000.011.1301328
TACTGGA39550.011.0297122
TTCGCGA5100.011.02937217
Produced by FastQC (version 0.11.5)